Xiang Ba, Li Yumei, Li Jianping, Zhang Baoyu, Li Jianyu, Jiang HuaiZhi, Zhang QiaoLing
College of Veterinary Medicine, Jilin University, Changchun, China.
College of Animal Science and Technology, Jilin University, Changchun, China.
Anim Biotechnol. 2023 Dec;34(9):4695-4702. doi: 10.1080/10495398.2023.2186891. Epub 2023 Mar 10.
Increasing Cashmere production can add value because it is the primary product of Cashmere goats. Recent years, peoples find miRNAs are crucial in regulating the development of hair follicle. Following Solexa sequencing, many miRNAs were distinguishingly expressed in telogen skin samples of goats and sheep in earlier study. But the method through which miR-21 controls the growth of hair follicles is still ambiguous. Bioinformatics analysis was used to predict the target genes of miR-21. The mRNA level of miR-21 in telogen Cashmere goat skins was higher than in anagen, according to the results of qRT-PCR, and the target genes expressed similarly with miR-21. Western blot showed similar trend, the protein expression of FGF18 and SMAD7 were lower in anagen samples. The Dual-Luciferase reporter assay confirmed miRNA-21's relationship with its target gene, and the consequences indicated found FGF18 and SMAD7 have positive correlations with miR-21. Western blot and qRT-PCR distinguished the expression of protein and mRNA in miR-21 and its target genes. According to the consequence, we found that target genes expression was increased by miR-21 in HaCaT cells. This study identified that miR-21 might take part in the development of Cashmere goat's hair follicles by targeting FGF18 and SMAD7.
提高羊绒产量可以增加其价值，因为羊绒是绒山羊的主要产品。近年来，人们发现微小RNA（miRNAs）在调节毛囊发育中起着关键作用。在早期研究中，通过Solexa测序发现，许多miRNAs在山羊和绵羊的休止期皮肤样本中差异表达。但miR-21调控毛囊生长的具体机制仍不明确。本研究利用生物信息学方法预测miR-21的靶基因。qRT-PCR结果显示，miR-21在绒山羊休止期皮肤中的mRNA水平高于生长期，且其靶基因的表达趋势与之相似。Western blot检测结果显示出类似趋势，生长期样本中FGF18和SMAD7的蛋白表达较低。双荧光素酶报告基因检测证实了miRNA-21与其靶基因的关系，结果表明FGF18和SMAD7与miR-21呈正相关。Western blot和qRT-PCR分别检测了miR-21及其靶基因的蛋白和mRNA表达。结果显示，在HaCaT细胞中，miR-21可上调其靶基因的表达。本研究表明，miR-21可能通过靶向FGF18和SMAD7参与绒山羊毛囊的发育。
