Student Research Committee, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Int J Environ Health Res. 2023 Oct;33(10):1010-1021. doi: 10.1080/09603123.2022.2067328. Epub 2022 Apr 21.
spp. cause keratitis and encephalitis, and are a proper carrier of foodborne pathogens. A total of 70 samples including garden cress, chives, mint, parsley, and basil were collected. Samples were cultured onto a 2% non-nutrient agar medium. The cultures were analyzed using morphological and molecular techniques. In total, 18 (25.7%) out of 70 samples were positive including garden cress 10/22 (45.45%), chives 3/12 (25%), mint 2/13 (15.38%), basil 2/13 (15.38%), and parsley 1/10 (10%). The diagnostic fragment 3 was successfully sequenced in 15 samples and represented 11 (73.3%) T4, three (20%) T5, and one T9 genotypes. In addition, three, two, and one strains, belonging to the genotypes T4, T5, and T9 were ranked highly pathogenic. This is the first study reporting contamination of the most commonly consumed fresh vegetables with pathogenic genotypes. Our findings signify the public health concerns due the contamination of vegetables in municipal public markets.
共采集了包括荠菜、韭菜、薄荷、欧芹和罗勒在内的 70 份样品。将样品接种于 2%非营养琼脂培养基上,采用形态学和分子技术进行分析。在 70 份样本中,有 18 份(25.7%)呈阳性,包括荠菜 10/22(45.45%)、韭菜 3/12(25%)、薄荷 2/13(15.38%)、罗勒 2/13(15.38%)和欧芹 1/10(10%)。成功对 15 个样本的诊断片段 3 进行了测序,代表 11 个(73.3%)T4、3 个(20%)T5 和 1 个 T9 基因型。此外,属于 T4、T5 和 T9 基因型的 3、2 和 1 株菌株被列为高致病性。这是首次报道市售常见食用鲜蔬受到致病性基因型污染的研究。我们的研究结果表明,由于市售蔬菜受到污染,存在公共卫生隐患。