Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao, China.
Research Center of Rural Vitalization, Hebei Normal University of Science and Technology, Qinhuangdao, China.
J Biomater Sci Polym Ed. 2022 Aug;33(12):1495-1510. doi: 10.1080/09205063.2022.2068942. Epub 2022 Apr 26.
Chitosan biomaterials are widely used in the biological area because of their broad-spectrum antibacterial activity. However, chitosan cannot be dissolved in a neutral solution, limiting its application in various fields seriously. In this study, water-soluble sulfonated oxidized chitosan (SOCS) with antifungal activity were prepared by oxidization and sulfonation. Its structure was clearly confirmed by spectroscopy data (FTIR, 1H NMR, C NMR) and elemental analysis. SEM images of OCS and SOCS revealed that there was a little curly and an irregular sheet-like morphologies on them which was attributed to the oxidation and sulfonation on CS. Moreover, the FTIR and NMR indicated that -OH on the CS was oxidized into -COOH on the OCS and -SOH groups on the SOCS. The EDS results of OCS and SOCS confirmed the presence of the oxygen element in OCS and the S element in SOCS. All studies confirmed the OCS and SOCS were synthesized successfully. Furthermore, the inhibitory activity of SOCS biocomposites against plant pathogenic fungi, (), was investigated. The results showed that the SOCS have significant inhibitory effects on the mycelial growth of . The EC value of SOCS against is 79.46 μg/mL. The research results presented above indicated that SOCS can be used as a candidate material for the control of plant pathogenic fungi, and can broaden the application of chitosan materials in plant protection and sustainable agriculture.Research highlightsSOCS showed better solubility in deionized water.The antifungal effect of SOCS dissolved in acetic acid was higher than that of CS dissolved in acetic acid.SOCS dissolved in water can cause an inhibitory effect on at lower concentrations.
壳聚糖生物材料由于具有广谱抗菌活性而广泛应用于生物领域。然而，壳聚糖不能溶解在中性溶液中，严重限制了其在各个领域的应用。本研究通过氧化和磺化制备了具有抗真菌活性的水溶性磺化氧化壳聚糖（SOCS）。其结构通过光谱数据（FTIR、1H NMR、C NMR）和元素分析得到了明确证实。OCS 和 SOCS 的 SEM 图像表明，它们表面有少量卷曲和不规则片状形态，这归因于 CS 的氧化和磺化。此外，FTIR 和 NMR 表明 CS 上的-OH 被氧化成 OCS 上的-COOH 和 SOCS 上的-SOH 基团。OCS 和 SOCS 的 EDS 结果证实了 OCS 中存在氧元素和 SOCS 中存在硫元素。所有研究都证实了 OCS 和 SOCS 的成功合成。此外，还研究了 SOCS 生物复合材料对植物病原真菌（）的抑制活性。结果表明，SOCS 对 的菌丝生长具有显著的抑制作用。SOCS 对 的 EC 值为 79.46μg/mL。上述研究结果表明，SOCS 可以用作控制植物病原真菌的候选材料，并可以拓宽壳聚糖材料在植物保护和可持续农业中的应用。研究亮点SOCS 在去离子水中具有更好的溶解性。SOCS 溶解在乙酸中的抗真菌效果高于 CS 溶解在乙酸中的效果。较低浓度的 SOCS 溶解在水中就能对 产生抑制作用。
