Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China.
Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Analysis and Testing Center, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China.
Int J Biol Macromol. 2022 Oct 31;219:1112-1121. doi: 10.1016/j.ijbiomac.2022.08.168. Epub 2022 Aug 29.
Fusarium graminearum (F. graminearum), a pathogen for Fusarium head blight (FHB) on wheat, significantly reduces wheat yield and poses potential threats to human food safety. In this study, graphene oxide (GO) modified chitosan (GO-CS composite) was synthesized and its antifungal activity against F. graminearum in vitro and in vivo was evaluated. The HNMR and FTIR results revealed the reaction between the carboxyl groups in GO and the amino groups in chitosan (CS). In vitro, the combination of GO and CS resulted in a significant synergistic inhibitory effect on the mycelial growth of F. graminearum relative to single GO or CS. The EC value of the GO-CS composite was 14.07 μg/mL, which was much lower than that of GO or CS alone. In vivo, the GO-CS composite significantly reduced the disease incidence and severity compared with single GO or CS, and the control efficacy could reach 60.01 %. Microbial cells might be ultimately damaged when interacting with GO-CS due to various mechanisms such as biological effects and physical barriers. Overall, the combination of GO and CS provides new opportunities for their application in the control of fungi.
禾谷镰刀菌（Fusarium graminearum）是一种引起小麦赤霉病（FHB）的病原菌，它严重降低了小麦的产量，并对人类食品安全构成潜在威胁。在本研究中，合成了氧化石墨烯（GO）修饰壳聚糖（GO-CS 复合材料），并评价了其对禾谷镰刀菌的体外和体内抗真菌活性。HNMR 和 FTIR 结果表明 GO 中的羧基基团与壳聚糖（CS）中的氨基基团发生了反应。体外实验中，GO 和 CS 的组合对禾谷镰刀菌的菌丝生长表现出显著的协同抑制作用，与单独的 GO 或 CS 相比。GO-CS 复合材料的 EC 值为 14.07μg/mL，远低于单独的 GO 或 CS。体内实验中，GO-CS 复合材料与单独的 GO 或 CS 相比，显著降低了病害发生率和严重程度，防治效果可达 60.01%。当微生物细胞与 GO-CS 相互作用时，可能会因生物效应和物理屏障等各种机制而最终受到损伤。总的来说，GO 和 CS 的组合为它们在真菌防治中的应用提供了新的机会。
