Ermolin G A, Efremov E E, Filimonova E V, Kotelianskiĭ V E, Voloshchuk O M
Vopr Med Khim. 1986 Nov-Dec;32(6):123-6.
Quantitative immunoenzyme assay of fibronectin in blood plasma is developed. Isolation and purification of fibronectin from human blood plasma, harvesting of specific antibodies to fibronectin, production of the antibodies and peroxidase conjugates and step-by-step procedure of the immunoenzyme assay in a "sandwich" modification are described. The assay was applied for diagnostics of several infectious and somatic diseases.
开展了血浆中纤连蛋白的定量免疫酶测定。描述了从人血浆中分离和纯化纤连蛋白、获取纤连蛋白特异性抗体、制备抗体和过氧化物酶结合物以及“夹心”改良免疫酶测定的分步程序。该测定法用于多种感染性疾病和躯体疾病的诊断。