Departamento de Medicina Veterinária, Universidade Federal de Viçosa, Av. P H Rolfs s/n, Viçosa, Minas Gerais, Brazil.
Departamento de Medicina Veterinária, Centro de Ciências Agrárias e Engenharias, Universidade Federal do Espírito Santo-UFES, Alegre, ES, Brazil.
Arch Microbiol. 2022 Apr 20;204(5):271. doi: 10.1007/s00203-022-02894-x.
Schistosoma mansoni is a heteroxenous parasite, meaning that during its life cycle needs the participation of obligatory intermediate and definitive hosts. The larval development occurs in aquatic molluscs belonging to the Biomphalaria genus, leading to the formation of cercariae, which emerge to infect the final vertebrate host. For this reason, studies for control of the diseases caused by digenetic trematodes often focus on combating the snail hosts. Thus, the objective of this study was to evaluate the susceptibility of Biomphalaria tenagophila embryos to the fungus Pochonia chlamydosporia (isolate Pc-10). The entire experiment was conducted in duplicate, with five replicates for each repetition (five egg masses/replicate), utilizing a total of 100 egg masses, with 20-30 eggs/egg mass. At the end of 15 days, the egg masses were evaluated under a stereomicroscope to analyze the hatching of B. tenagophila embryos in both experimental groups. After days of interaction, the exposure to the fungal hyphae bodies significantly impaired the viability of the B. tenagophila eggs, inhibiting the embryogenesis process by 83.7% in relation to the control group. Transmission and scanning electron microscopic images revealed relevant structural alterations in the egg masses exposed to the hyphae action of the fungus, interfering in the development and hatching of the young snails under analysis. These results indicate the susceptibility of B. tenagophila embryos to the fungus P. chlamydosporia (isolate Pc-10) and suggest the potential of Pc-10 to be used in the control of intermediate host, for its ovicidal capacity and for being an ecologically viable option, but in vivo experiments become necessary.
曼氏血吸虫是一种异宿主寄生虫，这意味着在其生命周期中需要有必要的中间宿主和终末宿主的参与。幼虫发育发生在属于双腔贝属的水生软体动物中，导致尾蚴的形成，尾蚴逸出以感染最终的脊椎动物宿主。因此，针对双腔科吸虫病的控制研究通常侧重于对抗螺宿主。因此，本研究的目的是评估 Biomphalaria tenagophila 胚胎对真菌 Pochonia chlamydosporia（分离株 Pc-10）的敏感性。整个实验进行了两次重复，每个重复有五个重复（每个重复 5 个卵团），总共使用了 100 个卵团，每个卵团有 20-30 个卵。在第 15 天结束时，在立体显微镜下评估卵团，以分析两个实验组中 B. tenagophila 胚胎的孵化情况。在几天的相互作用后，暴露于真菌菌丝体显著损害了 B. tenagophila 卵的活力，与对照组相比，抑制了胚胎发生过程 83.7%。透射和扫描电子显微镜图像显示，暴露于真菌菌丝体作用下的卵团发生了相关的结构改变，干扰了分析中幼螺的发育和孵化。这些结果表明 B. tenagophila 胚胎对真菌 P. chlamydosporia（分离株 Pc-10）的敏感性，并表明 Pc-10 具有作为杀卵剂和生态可行选择来控制中间宿主的潜力，但需要进行体内实验。
