Hutech Institute of Applied Science, HUTECH University, Ho Chi Minh, Vietnam.
Graduate University of Sciences and Technology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam.
Curr Microbiol. 2022 Apr 21;79(6):165. doi: 10.1007/s00284-022-02862-0.
To demonstrate that the amount of extracellular polymeric substances (EPS) and the freeze-dried viability of probiotics are correlated. Three strains of probiotics including Lactiplantibacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium bifidum were subjected to environmental challenges, such as temperature, pH, and carbon dioxide. The results indicated that the challenges could stimulate the EPS synthesis of the probiotics. The experimental correlation between the amount of synthesized EPS and the freeze-dried survival rate was also analyzed, and the viability of each of the three strains was represented by the following functions in which the equation of L. plantarum is y = - 0.0336x + 2.7059x - 14.849 with R = 0.9699, the B. bifidum's equation is y = - 0.0554x + 2.6243x - 13.654 with R = 0.9554, and the L. acidophilus's one was y = 0.0346x + 0.5862x - 9.1339 with R = 0.9733. This could be a new approach to determining the freeze-dried viability of probiotic strains based on the measured EPS content.
为了证明胞外聚合物（EPS）的含量和益生菌的冷冻干燥存活率之间存在相关性。选择了三株益生菌（植物乳杆菌、嗜酸乳杆菌和双歧杆菌）进行环境挑战，如温度、pH 值和二氧化碳。结果表明，这些挑战可以刺激益生菌的 EPS 合成。还分析了合成的 EPS 量与冷冻干燥存活率之间的实验相关性，并用以下函数表示三株菌的存活率，其中植物乳杆菌的方程为 y=-0.0336x+2.7059x-14.849，R=0.9699；双歧杆菌的方程为 y=-0.0554x+2.6243x-13.654，R=0.9554；嗜酸乳杆菌的方程为 y=0.0346x+0.5862x-9.1339，R=0.9733。这可能是一种基于测量 EPS 含量来确定益生菌冷冻干燥存活率的新方法。
