Department of Food and Nutrition, College of Natural Sciences, Myongji University, Cheoin-gu, Yongin, Gyeonggi-do 17058, Korea.
Rodney A. Erickson Food Science Building, Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USA.
Int J Med Mushrooms. 2022;24(2):63-74. doi: 10.1615/IntJMedMushrooms.2021042348.
Pleurotus ostreatus (PO) and Hericium erinaceus (HE) have been traditionally used to treat various diseases, owing to their antioxidant, antimicrobial, neuroprotective, and antitumor effects. However, few studies have been reported on their antiaging effects. In this study, the antioxidant and antiaging activities of PO and HE aqueous extracts were investigated in ultraviolet A (UVA)-induced human dermal fibroblast cells (HDFs). The antioxidant properties of PO and HE aqueous extracts were measured by total polyphenol and ergothioneine content, and their antioxidant activity was analyzed with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging assays. To demonstrate the antiaging effect of PO and HE aqueous extracts in UVA-induced HDFs, the secretion and mRNA expression of matrix metalloproteinase-1 (MMP-1), procollagen type I (PC1), and elastase were assessed by enzyme-linked immunosorbent assay (ELISA) and real-time PCR, respectively. The total polyphenol content in each extract was 13.6 and 11.7 mg gallic acid equivalents/g dry weight (DW), respectively, and the total ergothioneine content in each extract was 3.43 and 2.18 mg/g DW, respectively. The PO and HE extracts increased DPPH and ABTS radical-scavenging activity in a dose-dependent manner. In UVA-damaged HDFs, the extracts increased PC1 production but decreased MMP-1 production and elastase-1 activity. Furthermore, the mRNA levels of PC1, MMP-1, and elastase were recovered in the PO- and HE-treated UVA-irradiated HDFs compared to those in the irradiated control group. PO and HE aqueous extracts may be potentially used as a promising antiphotoaging agent.
糙皮侧耳（PO）和珊瑚状猴头菌（HE）因其具有抗氧化、抗菌、神经保护和抗肿瘤作用，已被传统用于治疗各种疾病。然而，关于它们的抗衰老作用的研究较少。在这项研究中，研究了 PO 和 HE 水提物在紫外线 A（UVA）诱导的人真皮成纤维细胞（HDF）中的抗氧化和抗衰老活性。通过总多酚和麦角硫因含量来测量 PO 和 HE 水提物的抗氧化特性，并通过 1,1-二苯基-2-苦基肼基（DPPH）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基清除测定来分析其抗氧化活性。为了证明 PO 和 HE 水提物在 UVA 诱导的 HDF 中的抗衰老作用，通过酶联免疫吸附测定（ELISA）和实时 PCR 分别评估基质金属蛋白酶-1（MMP-1）、I 型前胶原（PC1）和弹性蛋白酶的分泌和 mRNA 表达。每种提取物的总多酚含量分别为 13.6 和 11.7 mg 没食子酸当量/g 干重（DW），总麦角硫因含量分别为 3.43 和 2.18 mg/g DW。PO 和 HE 提取物以剂量依赖的方式增加 DPPH 和 ABTS 自由基清除活性。在 UVA 损伤的 HDF 中，提取物增加了 PC1 的产生，但降低了 MMP-1 的产生和弹性蛋白酶-1 的活性。此外，与照射对照组相比，PO 和 HE 处理的 UVA 照射 HDF 中的 PC1、MMP-1 和弹性蛋白酶的 mRNA 水平得到了恢复。PO 和 HE 水提物可能有望作为一种有前途的抗光老化剂。
