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新种,放线菌,从 (Lam.)的堆肥中分离得到。

sp. nov., an actinomycete isolated from compost of (Lam.).

机构信息

Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.

National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phaholyothin Road, Klong Luang, Pathum Thani, 12120, Thailand.

出版信息

Int J Syst Evol Microbiol. 2022 Apr;72(4). doi: 10.1099/ijsem.0.005320.

Abstract

A member of the genus , designated RCPT1-4, was isolated from compost of (Lam.), collected from an agricultural area in Rayong province, Thailand. The spore morphology and the presence of ll-diaminopimelic acid in the peptidoglycan indicate that RCPT1-4 shows the typical properties of members of the genus . On the basis of the results of 16S rRNA gene sequence analysis, the strain should be classified as representing a member of the genus and was most closely related to NBRC 12999 with the highest 16S rRNA gene sequence similarity of 99.2 %, followed by NBRC 14215 (99.0 %). In addition, RCPT1-4 shared the highest average nucleotide identity by blast (ANIb) (86.0 %), and digital DNA-DNA hybridization (dDDH) (32.1 %) values with NBRC 14215. Furthermore, several physiological and biochemical differences were observed between RCPT1-4 and the closely related type strains of species with validly published names. These taxonomic data indicated that RCPT1-4 could be considered to represent a novel species of the genus and the name sp. nov. is proposed for this strain. The type strain is RCPT1-4 (=TBRC 11260=NBRC 114303).

摘要

从泰国罗勇府一个农业区的堆肥中分离到一株属于的菌株,命名为 RCPT1-4。该菌株的孢子形态和肽聚糖中存在 ll-二氨基庚二酸表明其具有属的典型特征。基于 16S rRNA 基因序列分析的结果,该菌株应被归类为代表属的一个成员,与 NBRC 12999 的 16S rRNA 基因序列相似度最高,为 99.2%,其次是 NBRC 14215(99.0%)。此外,RCPT1-4 与 NBRC 14215 的平均核苷酸同一性blast(ANIb)(86.0%)和数字 DNA-DNA 杂交(dDDH)(32.1%)值最高。此外,RCPT1-4 与具有有效发表名称的相关种的近源模式株之间观察到了一些生理和生化差异。这些分类学数据表明,RCPT1-4 可以被认为是一个新的属种,建议将该菌株命名为 sp. nov.。该模式株为 RCPT1-4(=TBRC 11260=NBRC 114303)。

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