Duangupama Thitikorn, Intaraudom Chakapong, Pittayakhajonwut Pattama, Suriyachadkun Chanwit, Tadtong Sarin, Sirirote Pramote, Tanasupawat Somboon, Thawai Chitti
Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Klong Luang, Pathum Thani 12120, Thailand.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004857.
An actinobacterium, strain CH5-8, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of spp The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus were observed for strain CH5-8, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H), MK-9(H), and MK-9(H) as major menaquinones and anteiso-C, iso-C, and anteiso-C as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells. A combination of morphological and chemotaxonomic data supported the assignment to the genus . The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain. The novel strain CH5-8 showed the highest 16S rRNA gene sequence values to NBRC 12763 (98.9 %), followed by RCU-197 (98.9 %). The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8 and its closest relatives, CECT 3313 and RCU-197, were ≤91.6 % and ≤47.4 %, respectively. The digital DNA G+C content of genomic DNA was 72.1 mol%. On the basis of these phenotypic and genotypic data, strain CH5-8 represents a novel species, for which the name sp. nov. is proposed. The type strain is CH5-8 (=TBRC 9950=NBRC 113997).
从 spp 的根际土壤中分离出一株放线菌，菌株 CH5-8，它能从气生菌丝形成螺旋状孢子链。该菌株在 ISP2 培养基上呈现鲜黄绿色的基内菌丝，并易于产生中灰色的气生孢子团。菌株 CH5-8 具有该属成员典型的化学分类学特征，例如细胞肽聚糖中的 ll-二氨基庚二酸、主要的甲基萘醌为 MK-9(H)、MK-9(H)和 MK-9(H)，主要脂肪酸为 anteiso-C、iso-C 和 anteiso-C。在细胞中检测到了双磷脂酰甘油、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。形态学和化学分类学数据的组合支持将其归为该属。16S rRNA 基因序列分析结果证实了该菌株在属水平上的分类归属。新菌株 CH5-8 与 NBRC 12763（98.9%）的 16S rRNA 基因序列相似度最高，其次是 RCU-197（98.9%）。CH5-8 与其最接近的亲缘种 CECT 3313 和 RCU-197 之间的基于 blast 的平均核苷酸同一性（ANIb）和数字 DNA-DNA 杂交值分别≤91.6%和≤47.4%。基因组 DNA 的数字 DNA G+C 含量为 72.1 mol%。基于这些表型和基因型数据，菌株 CH5-8 代表一个新物种，为此提出新物种名称 sp. nov.。模式菌株为 CH5-8（=TBRC 9950=NBRC 113997）。
