Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin, China.
School of Zhang Zhongjing Health Care and Food, Nanyang Institute of Technology, Nanyang, China.
Foodborne Pathog Dis. 2022 May;19(5):304-310. doi: 10.1089/fpd.2021.0095. Epub 2022 Apr 21.
This study aimed to investigate the prevalence of in goat milk-based infant formula (GIF) collected from Shaanxi Province, China, and reveal the molecular characterization and antibiotic resistance profile of these isolates. A total of 750 GIF samples were collected from the retail markets in 5 cities in Shaanxi Province from February 2019 to February 2021. Molecular characterization was investigated using multilocus sequence typing and O-antigen serotyping. Antibiotic resistance of isolates was assessed using antimicrobial susceptibility testing. Thirty-two strains of were isolated from GIF samples with a prevalence rate of 4.27% and were divided into 16 sequence types (STs); among them, ST4 (6/32, 18.75%) and ST21 (5/32, 15.63%) were dominant. Five serotypes (O2, O1, O7, O4, and O3) were detected, and serotype O2 (15/32, 46.88%) was the main. Of the 21 antimicrobials, isolates showed higher resistance against cephalothin (87.5%), amoxicillin (25%), azithromycin (18.75%), oxytetracycline (18.75%), ampicillin (12.5%), and streptomycin (12.5%). In addition, three isolates were found to be resistant to three antimicrobials. These findings revealed the potential epidemiological risk and characterization of in GIF from Shaanxi Province, China, and provided reference data for the effective prevention and control of in powdered infant formula.
本研究旨在调查中国陕西省市售羊乳基婴儿配方食品（goat milk-based infant formula，GIF）中 污染情况，揭示这些分离株的分子特征和抗生素耐药谱。于 2019 年 2 月至 2021 年 2 月，从陕西省 5 个城市的零售市场采集了 750 份 GIF 样品。采用多位点序列分型（multilocus sequence typing）和 O 抗原血清型分型对分子特征进行了研究。采用药敏试验评估 分离株的抗生素耐药性。从 GIF 样品中分离出 32 株 ，检出率为 4.27%，分为 16 个序列型（sequence types，ST）；其中 ST4（6/32，18.75%）和 ST21（5/32，15.63%）占优势。检测到 5 种 血清型（O2、O1、O7、O4 和 O3），主要血清型为 O2（15/32，46.88%）。在 21 种抗生素中，分离株对头孢噻吩（cephalothin，87.5%）、阿莫西林（amoxicillin，25%）、阿奇霉素（azithromycin，18.75%）、氧四环素（oxytetracycline，18.75%）、氨苄西林（ampicillin，12.5%）和链霉素（streptomycin，12.5%）的耐药率较高。此外，有 3 株分离株对 3 种抗生素耐药。这些发现揭示了中国陕西省市售 GIF 中 存在的潜在流行病学风险和特征，为有效防控粉状婴儿配方食品中 提供了参考数据。
