Blanks Anson M, Pedersen Lauren N, Bohmke Natalie, Mihalick Virginia L, Franco R Lee
Department of Kinesiology and Health Sciences, College of Humanities and Sciences, Virginia Commonwealth University, Richmond, VA, United States.
Department of Radiation Oncology, Washington University School of Medicine in St. Louis, St. Louis, MO, United States.
Life Sci. 2022 Jun 15;299:120557. doi: 10.1016/j.lfs.2022.120557. Epub 2022 Apr 19.
Monocyte chemokine receptor 2 (CCR2) and phosphorylated extra-cellular regulated kinase 1 & 2 (ERK1/2) impact macrophage differentiation and progression of atherosclerosis. Whereas aerobic exercise favorably modulates the immune system and reduces atherosclerotic risk, it is unknown whether sex differences exist in the monocyte/macrophage response to acute aerobic exercise.
To determine the impact of an acute bout of moderate intensity aerobic exercise on monocyte and macrophage CCR2 expression, ERK1/2 phosphorylation, and macrophage polarization in pre-menopausal women and men.
Blood samples were collected in 24 people (Women/Men; n = 12) prior to (PRE), immediately after a bout of moderate intensity cycle ergometry (POST), and 2 h (2H) following exercise. Monocyte and macrophage CCR2 and phosphorylated ERK1/2 as well as macrophage CD86 and CD206 were analyzed by flow cytometry.
PRE classical monocyte CCR2 expression was greater in women compared to men (Women: 20546.2 ± 2306.4 vs. Men: 14437.6 ± 1201.9 AUF; p = 0.028) and was reduced in women at 2H (PRE: 20546.2 ± 2306.4 vs. 2H: 15856.9 ± 1314.4 AUF; p = 0.027). POST classical monocyte CCR2 expression was inversely associated (r = -0.697, p = 0.012) with POST classical monocyte ERK1/2 phosphorylation in women only. The percentage of CCR2 macrophages was lower in women at POST (Women: 62.0 ± 8.9 vs. Men: 83.6 ± 3.1; p = 0.031) and at 2H (Women: 60.3 ± 8.4 vs. Men: 83.5 ± 3.0%; p = 0.016).
These data suggest that a single bout of moderate intensity aerobic exercise differentially impacts monocyte CCR2 expression and macrophage polarization in women compared to men.
单核细胞趋化因子受体2（CCR2）和磷酸化细胞外调节激酶1和2（ERK1/2）影响巨噬细胞分化和动脉粥样硬化进展。尽管有氧运动可有益地调节免疫系统并降低动脉粥样硬化风险，但单核细胞/巨噬细胞对急性有氧运动的反应是否存在性别差异尚不清楚。
确定一次急性中等强度有氧运动对绝经前女性和男性单核细胞及巨噬细胞CCR2表达、ERK1/2磷酸化以及巨噬细胞极化的影响。
采集24人（女性/男性；n = 12）的血样，分别在运动前（PRE）、一次中等强度蹬车运动后即刻（POST）以及运动后2小时（2H）采集。通过流式细胞术分析单核细胞和巨噬细胞的CCR2、磷酸化ERK1/2以及巨噬细胞CD86和CD206。
运动前，女性经典单核细胞CCR2表达高于男性（女性：20546.2 ± 2306.4 vs. 男性：14437.6 ± 1201.9 AUF；p = 0.028），且女性在运动后2小时该表达降低（运动前：20546.2 ± 2306.4 vs. 运动后2小时：15856.9 ± 1314.4 AUF；p = 0.027）。仅在女性中，运动后经典单核细胞CCR2表达与运动后经典单核细胞ERK1/2磷酸化呈负相关（r = -0.697，p = 0.012）。运动后即刻，女性CCR2巨噬细胞百分比低于男性（女性：62.0 ± 8.9 vs. 男性：83.6 ± 3.1；p = 0.031），运动后2小时亦是如此（女性：60.3 ± 8.4 vs. 男性：83.5 ± 3.0%；p = 0.016）。
这些数据表明，一次急性中等强度有氧运动对女性单核细胞CCR2表达和巨噬细胞极化的影响与男性不同。
