Graduate Program in Cellular and Molecular Biology Applied to Health, Lutheran University of Brazil, Canoas, RS, Brazil.
Master's Program in Health Promotion, Human Development, and Society, Lutheran University of Brazil, Canoas, RS, Brazil.
Acta Histochem. 2022 May;124(4):151894. doi: 10.1016/j.acthis.2022.151894. Epub 2022 Apr 18.
This study aimed to evaluate the efficacy and safety of a topical and oral administration of pumpkin seed oil (PSO) on the hair growth of BALB/c male mice. The animals had their dorsal area shaved (2 ×2 cm) and they were divided into 6 experimental groups. They received orally saline (OS), finasteride (F), or PSO (OP) for 14 days; or topically saline (TS), minoxidil (M), or PSO (TP) for 7 days. The euthanasia of all of the mice occurred on the 22nd day, and the histological slides from the skin area were analyzed. Lipoperoxidation in the liver was assessed through the TBARS method and was also evaluated by the antioxidant enzymes (SOD and CAT). The comet assay and the micronucleus tests were performed for genotoxic/mutagenic safety analyses. A significant increase in the number of hair follicles in the TP group was seen (8.8 ± 0.8) but it was disorganized, with loose dermal collagen. Finasteride presented a significant increase in the levels of the TBARS, SOD, and CAT in the liver, and M increased the DNA damage in the blood and the liver tissues. PSO did not induce any significant changes. In addition, PSO did not induce genotoxic or mutagenic effects. In conclusion, the oral PSO for 14 days acted in the proliferation of the hair follicles, without toxicity signals in the liver. DATA AVAILABILITY: The authors confirm that all of the relevant data is included in the article and/or in the supplementary information file.
本研究旨在评估南瓜籽油（PSO）经皮和口服给药对 BALB/c 雄性小鼠毛发生长的疗效和安全性。动物背部区域剃毛（2×2cm），并分为 6 个实验组。它们连续 14 天口服生理盐水（OS）、非那雄胺（F）或 PSO（OP）；或连续 7 天经皮给予生理盐水（TS）、米诺地尔（M）或 PSO（TP）。所有小鼠均在第 22 天处死，分析皮肤区域的组织学切片。通过 TBARS 法评估肝脏脂质过氧化作用，并通过抗氧化酶（SOD 和 CAT）进行评估。彗星试验和微核试验用于遗传毒性/诱变安全性分析。TP 组的毛囊数量显著增加（8.8±0.8），但组织松散，真皮胶原疏松。非那雄胺可显著增加肝脏中 TBARS、SOD 和 CAT 的水平，M 可增加血液和肝组织中的 DNA 损伤。PSO 没有引起任何显著变化。此外，PSO 没有诱导遗传毒性或致突变作用。总之，口服 PSO 连续 14 天作用于毛囊增殖，肝脏无毒性信号。数据可用性：作者确认所有相关数据均包含在文章中和/或补充信息文件中。
