Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Ecotoxicol Environ Saf. 2022 Jun 1;237:113518. doi: 10.1016/j.ecoenv.2022.113518. Epub 2022 Apr 18.
Ammonia nitrogen, a major oxygen-consuming pollutant in the environment, can adversely affect aquatic organisms such as fish, bivalves, and crustaceans. We investigated the toxic effects of ammonia nitrogen on the Hong Kong oyster, Crassostrea hongkongensis, using flow cytometry and H nuclear magnetic resonance metabolomics. Exposure to ammonia nitrogen caused time- and concentration-dependent alterations in various immune parameters in hemocytes and impaired the metabolic profiles of the gills. We observed changes in the rate of apoptosis, esterase activity, lysosomal mass, hemocyte counts, phagocytic activity, and mitochondrial mass. Exposure affected metabolic pathways involved in energy metabolism, osmotic balance, and oxidative stress. We concluded that ammonia nitrogen induces metabolic and hematological dysfunction in C. hongkongensis, and our findings provide insights into the biochemical defense strategies of bivalves exposed to acute high-concentration ammonia nitrogen.
氨氮是环境中主要的耗氧污染物，会对鱼类、双壳类和甲壳类等水生生物产生不利影响。我们使用流式细胞术和 H 核磁共振代谢组学研究了氨氮对香港牡蛎（Crassostrea hongkongensis）的毒性作用。暴露于氨氮会导致血细胞中各种免疫参数随时间和浓度的变化，并损害鳃的代谢谱。我们观察到细胞凋亡率、酯酶活性、溶酶体质量、血细胞计数、吞噬活性和线粒体质量的变化。暴露会影响参与能量代谢、渗透平衡和氧化应激的代谢途径。我们得出结论，氨氮会导致 C. hongkongensis 的代谢和血液功能障碍，我们的研究结果为了解贝类暴露于急性高浓度氨氮时的生化防御策略提供了线索。
