Chen Shubing, Li Shuang, Fang Keyi, Wang Yongjian, Yang Yan, Han Chao, Shen Yan
Technical Center of Ningbo Customs, Ningbo 315040, China.
Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
Food Chem. 2022 Sep 15;388:132976. doi: 10.1016/j.foodchem.2022.132976. Epub 2022 Apr 15.
Banned industrial dyes are composed of a large number of chemicals with diverse physical and chemical properties, making their simultaneous determination a challenging task. A one-step extraction and purification of 93 banned industrial dyes from beverage, fish and cookie sample methods was proposed by using solid supported liquid-liquid extraction (SLE). The extract was analyzed by ultrahigh-performance liquid chromatography quadrupole orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap-HRMS). The quantitative and qualitative mode adopts Q-Orbitrap-HRMS full scan MS (full scan MS) and data-dependent MS/MS (dd-MS) acquisition mode. The mass resolution was screened under 70,000 FWHM for full-scan MS and 35,000 FWHM for dd-MS. Linearity was observed in the range of 0.01 ∼ 0.5 μg/mL and the limits of quantification were 0.04 ∼ 0.2 mg/kg for 93 dyes. The average recoveries were 70.5-105.8%, with RSD ≤ 10%. The proposed method has the ability to simultaneously screen many banned dyes in foods with high throughput, sensitivity and reliability.
禁用工业染料由大量具有不同物理和化学性质的化学物质组成，这使得同时测定它们成为一项具有挑战性的任务。提出了一种使用固相支撑液液萃取（SLE）从饮料、鱼类和饼干样品中一步萃取和纯化93种禁用工业染料的方法。提取物通过超高效液相色谱四极杆轨道阱高分辨率质谱（UPLC-Q-Orbitrap-HRMS）进行分析。定量和定性模式采用Q-Orbitrap-HRMS全扫描质谱（全扫描MS）和数据依赖型二级质谱（dd-MS）采集模式。全扫描MS的质量分辨率在70,000半高宽下筛选，dd-MS的质量分辨率在35,000半高宽下筛选。在0.01 ∼ 0.5 μg/mL范围内观察到线性关系，93种染料的定量限为0.04 ∼ 0.2 mg/kg。平均回收率为70.5-105.8%，相对标准偏差≤10%。所提出的方法能够以高通量、高灵敏度和高可靠性同时筛选食品中的多种禁用染料。
