Guangzhou Institute for Food Inspection, Guangzhou 511410, China.
Key Laboratory of Molecular Target & Clinical Pharmacology, School of Pharmaceutical Sciences & the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, China.
Food Chem. 2023 Nov 30;427:136665. doi: 10.1016/j.foodchem.2023.136665. Epub 2023 Jun 17.
Public exposure to synthetic dyes through foods has attracted ongoing and serious attention. Here we developed and validated a simultaneous screening and quantitation method for the analysis of fat-soluble synthetic dyes that most frequently found in foods, using C d-SPE clean-up and UHPLC-Q-Orbitrap HRMS on Full-MS/dd-MS mode. During a single run, 104 dyes including 6 pairs of isomers were distinguished based on chromatographic separation and unique product ions. The method showed satisfactory linearity (R > 0.99), recoveries (61.3 %-118.8 %), precision (<20 %) and limit of quantification (0.05-0.5 mg/kg). For 98 % of test dyes, screening detection limits ranged from 2.5 to 25 μg/kg. The validated method was successfully applied to real commercial foodstuffs revealing the presence of two selected illegal dyes in three samples.
公众通过食物摄入合成染料的问题引起了持续而严重的关注。在这里，我们开发并验证了一种同时筛选和定量分析脂溶性合成染料的方法，这些染料是食品中最常见的，采用 C d-SPE 净化和 UHPLC-Q-Orbitrap HRMS 在全扫描/数据依赖扫描模式下进行分析。在单次运行中，基于色谱分离和独特的产物离子，可区分 104 种染料，包括 6 对异构体。该方法表现出良好的线性（R＞0.99）、回收率（61.3％-118.8％）、精密度（＜20％）和定量限（0.05-0.5mg/kg）。对于 98％的测试染料，筛选检测限范围为 2.5 至 25μg/kg。该验证方法成功应用于实际商业食品中，在三个样品中发现了两种选定的非法染料。
