United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Wheat, Peanut, and Other Field Crop Research Unit, Stillwater, OK 74075.
United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Hard Winter Wheat Genetics Research Unit, Manhattan, KS 66506.
Phytopathology. 2021 Apr;111(4):649-658. doi: 10.1094/PHYTO-07-20-0308-R. Epub 2021 Mar 24.
Leaf rust, caused by , is one of the most common wheat () diseases in the Great Plains of the United States. A population of recombinant inbred lines from CI 17884 × 'Bainong 418' was evaluated for responses to leaf rust race - and genotyped using single nucleotide polymorphism (SNP) markers. Quantitative trait locus analysis identified a minor gene for resistance to leaf rust, designated .-, on the 1BL.1RS translocation segment in 'Bainong 418', and another leaf rust resistance gene, , on chromosome 7A of CI 17884. , originally identified in CI 17884 and located in a wheat-. translocation segment 7S#1S, remains one of only a few race-specific resistance genes still effective in the Great Plains. A set of 7A-specific simple sequence repeat markers were developed and used to genotype CI 17884 and a pair of near-isogenic lines differing in the presence or absence of 7S#1S, PI 603918, and 'Pavon F76'. Haplotype analysis indicated that the estimated length of 7S#1S was 157.23 to 174.42 Megabases, accounting for ∼23% of the 7A chromosome. Two SNPs on 7S#1S and four SNPs on the 1RS chromosome arm were converted to Kompetitive allele-specific PCR (KASP) markers, which were subsequently validated in a panel of cultivars and elite breeding lines released within the last decade. Of these, one- and two-KASP markers are specific to the 1RS chromosome arm and 7S#1S, respectively, indicating that they can facilitate the introgression of and .- into locally adapted wheat cultivars and breeding lines.
叶锈病，由 引起，是美国大平原地区最常见的小麦病害之一。来自 CI 17884×'Bainong 418'的重组自交系群体被评估对叶锈病的反应，并使用单核苷酸多态性（SNP）标记进行基因分型。数量性状位点分析在 'Bainong 418'的 1BL.1RS 易位片段上鉴定了一个对叶锈病的抗性的小基因，命名为 。-，在 CI 17884 的 7A 染色体上鉴定了另一个叶锈病抗性基因 。-。最初在 CI 17884 中鉴定出的 ，位于小麦- 易位片段 7S#1S 中，仍然是大平原地区仅有的少数几个有效、针对特定品种的抗性基因之一。开发了一套 7A 特异性简单重复序列标记，并用于对 CI 17884 及其一对在是否存在 7S#1S、PI 603918 和 'Pavon F76'方面存在差异的近等基因系进行基因分型。单倍型分析表明，7S#1S 的估计长度为 157.23 至 174.42 兆碱基，占 7A 染色体的约 23%。7S#1S 上的两个 SNP 和 1RS 染色体臂上的四个 SNP 被转换为 Kompetitive allele-specific PCR（KASP）标记，随后在过去十年中发布的一组品种和优良育种系中进行了验证。其中，一个和两个 KASP 标记分别针对 1RS 染色体臂和 7S#1S 是特异性的，表明它们可以促进 和 。- 导入到当地适应的小麦品种和育种系中。
