Xu Xin, Meng Xiangkun, Zhang Nan, Jiang Heng, Ge Huichen, Qian Kun, Wang Jianjun
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
Pestic Biochem Physiol. 2021 Aug;177:104905. doi: 10.1016/j.pestbp.2021.104905. Epub 2021 Jun 24.
The sulfuryl transfer reaction catalyzed by cytosolic sulfotransferase (SULT) is one of the major conjugating pathways responsible for the detoxification and subsequent elimination of xenobiotics, however, functional characterization of insect SULTs is still limited. In this study, cDNA encoding a cytosolic sulfotransferase, named TcSULT1, was cloned from the red flour beetle, Tribolium castaneum. Sequence analysis revealed that TcSULT1 had the conserved signature sequences of SULTs, and shared moderate amino acid identities with Bombyx mori and Drosophila SULTs. Analysis of the transcription level showed that TcSULT1 was highly expressed in head, epidermis and malpighian tube, and upregulated at 4 h after exposure to deltamethrin. Knockdown of TcSULT1 significantly increased the susceptibility of beetles to deltamethrin. Both RNAi and dual-luciferase assay revealed that the transcription factor TcCncC regulates the expression of TcSULT1. These data provides insights into the function and regulatory mechanism of insect SULTs.
胞质磺基转移酶（SULT）催化的磺酰基转移反应是负责外源性物质解毒和后续消除的主要结合途径之一，然而，昆虫SULTs的功能特性仍很有限。在本研究中，从赤拟谷盗（Tribolium castaneum）中克隆了编码一种胞质磺基转移酶的cDNA，命名为TcSULT1。序列分析表明，TcSULT1具有SULTs的保守特征序列，与家蚕和果蝇的SULTs具有中等程度的氨基酸同一性。转录水平分析表明，TcSULT1在头部、表皮和马氏管中高表达，并在接触溴氰菊酯后4小时上调。敲低TcSULT1显著增加了甲虫对溴氰菊酯的敏感性。RNA干扰和双荧光素酶测定均表明转录因子TcCncC调节TcSULT1的表达。这些数据为昆虫SULTs的功能和调控机制提供了见解。
