Yu Haizhong, Yi Long, Lu Zhanjun
College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou 341000, China.
Insects. 2022 Apr 2;13(4):353. doi: 10.3390/insects13040353.
Chitin is a major component of the arthropod exoskeleton, always working together with chitin-binding proteins to maintain the functions of extracellular structures. In the present study, we identified a cuticle protein 64 from using a chitin-binding assay. Bioinformatics analysis revealed that DcCP64 contained eight conserved PYPV motifs but lacked a Rebers-Riddiford (R-R) consensus and other chitin-binding domains. RT-qPCR analysis suggested that had the highest expression level in the wing and fifth-instar nymph stage. Knockdown of by RNA interference (RNAi) resulted in a malformed-wing phenotype, higher mortality and decreased molting rate. Furthermore, transcriptomics analysis revealed that 1244 differentially expressed genes (DEGs) were up-regulated and 580 DEGs were down-regulated, compared with ds groups and ds groups. KEGG enrichment analysis revealed that up-regulated DEGs were mainly related to oxidative phosphorylation, whereas down-regulated DEGs were mainly involved in the MAPK and FoxO signaling pathways. Moreover, inhibition of significantly affected the cuticle surface, and increased the permeability of the abdomen and wings. Further chitin- and cellulose-binding assay confirmed the chitin-binding properties of recombinant DcCP64 in vitro. These results indicate that might play an important role in the cuticle and wing development of .
几丁质是节肢动物外骨骼的主要成分，总是与几丁质结合蛋白协同作用以维持细胞外结构的功能。在本研究中，我们通过几丁质结合试验从[具体来源未给出]中鉴定出一种表皮蛋白64。生物信息学分析表明，DcCP64包含八个保守的PYPV基序，但缺乏Rebers-Riddiford（R-R）共有序列和其他几丁质结合结构域。RT-qPCR分析表明，[具体基因未给出]在翅和五龄若虫阶段表达水平最高。通过RNA干扰（RNAi）敲低[具体基因未给出]导致翅畸形表型、更高的死亡率和降低的蜕皮率。此外，转录组学分析显示，与ds对照组和ds对照组相比，有1244个差异表达基因（DEG）上调，580个DEG下调。KEGG富集分析表明，上调的DEG主要与氧化磷酸化有关，而下调的DEG主要参与MAPK和FoxO信号通路。此外，抑制[具体基因未给出]显著影响表皮表面，并增加腹部和翅膀的通透性。进一步的几丁质和纤维素结合试验在体外证实了重组DcCP64的几丁质结合特性。这些结果表明，[具体基因未给出]可能在[具体物种未给出]的表皮和翅发育中起重要作用。
