College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi Province, China.
National Navel Orange Engineering Research Center, Ganzhou, Jiangxi Province, China.
Insect Sci. 2021 Dec;28(6):1690-1707. doi: 10.1111/1744-7917.12880. Epub 2020 Nov 26.
Validamycin has been widely used as a specific competitive inhibitor of trehalase. In our previous research, validamycin significantly inhibited trehalase activity and chitin synthesis in Diaphorina citri, resulting in abnormal phenotypes. However, the mechanism of validamycin's action on D. citri remains unclear. Here, using a comparative transcriptome analysis, 464 differentially expressed genes (DEGs) in D. citri were identified after validamycin treatment. A Gene Ontology enrichment analysis revealed that these DEGs were mainly involved in "small molecule process", "structural molecule activity" and "transition metal ion binding". DEGs involved in chitin metabolism, cuticle synthesis and insecticide detoxification were validated by reverse transcription quantitative polymerase chain reaction. The RNA interference of D. citri chitinase-like protein ENO3 and D. citri cuticle protein 7 genes significantly affected D. citri molting. Moreover, the recombinant chitinase-like protein ENO3 exhibited a chitin-binding property, and an antimicrobial activity against Bacillus subtilis. This study provides a first insight into the molecular changes in D. citri after exposure to validamycin and identifies two effective RNA interference targets for D. citri control.
井冈霉素作为一种专一性的海藻糖酶竞争性抑制剂被广泛应用。在我们之前的研究中，井冈霉素显著抑制了柑橘木虱海藻糖酶的活性和几丁质的合成，导致了异常的表型。然而，井冈霉素对柑橘木虱的作用机制仍不清楚。在这里，通过比较转录组分析，在井冈霉素处理后，鉴定出柑橘木虱中 464 个差异表达基因（DEGs）。GO 富集分析表明，这些 DEGs 主要参与“小分子过程”、“结构分子活性”和“过渡金属离子结合”。通过反转录定量聚合酶链反应验证了参与几丁质代谢、表皮合成和杀虫剂解毒的 DEGs。柑橘木虱几丁质酶样蛋白 ENO3 和柑橘木虱表皮蛋白 7 基因的 RNA 干扰显著影响了柑橘木虱的蜕皮。此外，重组几丁质酶样蛋白 ENO3 表现出几丁质结合特性，并对枯草芽孢杆菌具有抗菌活性。本研究首次揭示了柑橘木虱暴露于井冈霉素后的分子变化，并确定了两种有效的柑橘木虱 RNAi 干扰靶点。
