College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
National Navel Orange Engineering Research Center, Ganzhou 341000, China.
Int J Mol Sci. 2022 Aug 25;23(17):9654. doi: 10.3390/ijms23179654.
Glycogen is a predominant carbohydrate reserve in various organisms, which provides energy for different life activities. Glycogen synthase kinase 3 (GSK3) is a central player that catalyzes glucose and converts it into glycogen. In this study, a gene was identified from the genome database and named . A reverse transcription quantitative PCR (RT-qPCR) analysis showed that was expressed at a high level in the head and egg. The silencing of by RNA interference (RNAi) led to a loss-of-function phenotype. In addition, knockdown decreased trehalase activity, glycogen, trehalose, glucose and free fatty acid content. Moreover, the expression levels of the genes associated with chitin and fatty acid synthesis were significantly downregulated after the silencing of . According to a comparative transcriptomics analysis, 991 differentially expressed genes (DEGs) were identified in ds groups compared with ds groups. A KEGG enrichment analysis suggested that these DEGs were primarily involved in carbon and fatty acid metabolism. The clustering analysis of DEGs further confirmed that chitin and fatty acid metabolism-related DEGs were upregulated at 24 h and were downregulated at 48 h. Our results suggest that plays an important role in regulating the chitin and fatty acid metabolism of .
糖原是各种生物中主要的碳水化合物储备，为不同的生命活动提供能量。糖原合酶激酶 3（GSK3）是一种重要的酶，它可以催化葡萄糖并将其转化为糖原。本研究从 基因组数据库中鉴定出一个基因，并将其命名为 。反转录定量 PCR（RT-qPCR）分析表明， 在头部和卵中表达水平较高。通过 RNA 干扰（RNAi）沉默 导致功能丧失表型。此外， 的敲低降低了海藻糖酶活性、糖原、海藻糖、葡萄糖和游离脂肪酸的含量。此外，沉默 后与几丁质和脂肪酸合成相关的基因表达水平显著下调。根据比较转录组学分析，ds 组与 ds 组相比，有 991 个差异表达基因（DEGs）。KEGG 富集分析表明，这些 DEGs 主要参与碳和脂肪酸代谢。DEGs 的聚类分析进一步证实，几丁质和脂肪酸代谢相关的 DEGs 在 24 小时时上调，在 48 小时时下调。我们的结果表明， 在调节 的几丁质和脂肪酸代谢中起重要作用。
