"TheBlueChemistryLab", Department of Pharmacy, Task Force "BIGFED2", University of Naples Federico II, Via Domenico Montesano 49, 80131 Napoli, Italy.
Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Napoli, Italy.
Mar Drugs. 2022 Apr 6;20(4):256. doi: 10.3390/md20040256.
In this study, a strain of UTEX 1444 was cultivated in six different concentrations of iron (III). Cultures were extracted with organic solvents and analyzed using our dereplication strategy, based on the combined use of high-resolution tandem mass spectrometry and molecular networking. The analysis showed the presence of the siderophores' family, named synechobactins, only in the zero iron (III) treatment culture. Seven unknown synechobactin variants were present in the extract, and their structures have been determined by a careful HRMS/MS analysis. This study unveils the capability of UTEX 1444 to produce a large array of siderophores and may be a suitable model organism for a sustainable scale-up exploitation of such bioactive molecules, for the bioremediation of contaminated ecosystems, as well as in drug discovery.
在这项研究中，将一株 UTEX 1444 在六种不同浓度的三价铁（III）中进行培养。用有机溶剂提取培养物，并使用我们的去重策略进行分析，该策略基于高分辨率串联质谱和分子网络的联合使用。分析表明，只有在零铁（III）处理的培养物中存在铁载体家族，称为“蓝藻菌素”。在提取物中存在七种未知的蓝藻菌素变体，它们的结构通过仔细的高分辨率 MS/MS 分析确定。这项研究揭示了 UTEX 1444 生产大量铁载体的能力，它可能是一种合适的模式生物，可用于可持续扩大此类生物活性分子的规模生产，用于受污染生态系统的生物修复，以及药物发现。
