School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, China.
J Sep Sci. 2012 Sep;35(17):2313-7. doi: 10.1002/jssc.201200231. Epub 2012 Jul 17.
A rapid and efficient method for the separation and purification of fucoxanthin from edible brown algae by microwave-assisted extraction coupled with high-speed countercurrent chromatography was developed. The algae were first extracted using microwave-assisted extraction, then the dried extract was dissolved and directly introduced into the high-speed countercurrent chromatography system with a two-phase solvent system consisting of hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v/v/v). The isolation was done in less than 75 min, and a total of 0.83 mg, 1.09 mg, and 0.20 mg fucoxanthin were obtained from 25.0 g fresh Laminaria japonica Aresch, 1.5 g dry Undaria pinnatifida (Harv) Sur, and 15.0 g dry Sargassum fusiforme (Harv) Setch, respectively. The purity of fucoxanthin determined by HPLC was over 90% and its structure was further identified by LC-ESI-MS and (1) H-NMR.
建立了一种从食用褐藻中通过微波辅助提取结合高速逆流色谱快速高效分离纯化岩藻黄质的方法。首先用微波辅助提取法提取藻类，然后将干燥的提取物溶解并直接用正己烷-乙酸乙酯-乙醇-水（5:5:6:4，v/v/v/v）两相溶剂系统引入高速逆流色谱系统。分离在不到 75 分钟内完成，从 25.0 克新鲜的裙带菜、1.5 克干的昆布和 15.0 克干的羊栖菜中分别获得了 0.83mg、1.09mg 和 0.20mg 的岩藻黄质。HPLC 测定的岩藻黄质纯度超过 90%，其结构进一步通过 LC-ESI-MS 和（1）H-NMR 鉴定。
