Chasapis Christos T, Kelaidonis Konstantinos, Ridgway Harry, Apostolopoulos Vasso, Matsoukas John M
NMR Facility, Instrumental Analysis Laboratory, School of Natural Sciences, University of Patras, 26504 Patras, Greece.
Institute of Chemical Engineering Sciences, Foundation for Research and Technology, Hellas (FORTH/ICE-HT), 26504 Patras, Greece.
Brain Sci. 2022 Mar 24;12(4):434. doi: 10.3390/brainsci12040434.
Myelin in humans is composed of about 80% lipids and 20% protein. Initially, myelin protein composition was considered low, but various recent proteome analyses have identified additional myelin proteins. Although, the myelin proteome is qualitatively and quantitatively identified through complementary proteomic approaches, the corresponding Protein-Protein Interaction (PPI) network of myelin is not yet available. In the present work, the PPI network was constructed based on available experimentally supported protein interactions of myelin in PPI databases. The network comprised 2017 PPIs between 567 myelin proteins. Interestingly, structure-based in silico analysis revealed that 20% of the myelin proteins that are interconnected in the proposed PPI network are metal-binding proteins/enzymes that construct the main sub-PPI network of myelin proteome. Finally, the PPI networks of the myelin proteome and sub-metalloproteome were analyzed ontologically to identify the biochemical processes of the myelin proteins and the interconnectivity of myelin-associated diseases in the interactomes. The presented PPI dataset could provide a useful resource to the scientific community to further our understanding of human myelin biology and serve as a basis for future studies of myelin-related neurological diseases and particular autoimmune diseases such as multiple sclerosis where myelin epitopes are implicated.
人类髓磷脂约由80%的脂质和20%的蛋白质组成。最初，人们认为髓磷脂的蛋白质组成比例较低，但最近的各种蛋白质组分析已鉴定出了其他髓磷脂蛋白。尽管通过互补蛋白质组学方法在定性和定量方面鉴定了髓磷脂蛋白质组，但髓磷脂相应的蛋白质-蛋白质相互作用（PPI）网络尚不可用。在本研究中，基于PPI数据库中髓磷脂可用的实验支持的蛋白质相互作用构建了PPI网络。该网络包含567种髓磷脂蛋白之间的2017个PPI。有趣的是，基于结构的计算机模拟分析表明，在所提出的PPI网络中相互连接的髓磷脂蛋白中有20%是构建髓磷脂蛋白质组主要子PPI网络的金属结合蛋白/酶。最后，对髓磷脂蛋白质组和亚金属蛋白质组的PPI网络进行了本体分析，以确定髓磷脂蛋白的生化过程以及相互作用组中髓磷脂相关疾病的关联性。所呈现的PPI数据集可为科学界提供有用资源，以增进我们对人类髓磷脂生物学的理解，并为未来研究与髓磷脂相关的神经疾病以及特定自身免疫性疾病（如涉及髓磷脂表位的多发性硬化症）奠定基础。
