Svobodová Jana, Mikšík Ivan
Department of Translation Metabolism, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic.
Gels. 2022 Mar 22;8(4):198. doi: 10.3390/gels8040198.
Ethanol extracts of three widely growing plants were added to silica sol-gel solutions, which were subsequently applied as wall surface modifiers in inner quartz capillaries. Modified capillaries were used for open-tubular capillary electrochromatographic separation of nucleotides and amino groups containing biological compounds (neurotransmitters, amino acids and oligopeptides). The experiments were performed at physiological pH 7.40, and eventual changes of effective mobilities were calculated. Specific compounds characteristic for each plant were tested as sol-gel additives as well, and thus-modified capillaries were used for the separations of the same analytes under identical conditions. The aim of this study was to find out possible interactions between physiological compounds and extracts of freely available plants anchorded in the sol-gel stationary phase in the flowing system. Even though the amount of the modifier in each capillary is very small, basic statistical evaluation showed some not negligible changes in effective mobility of tested analytes. These changes were bigger than ±5% for separations of nucleotides in capillaries with curcuma, Moringa or the mixture of synthetic additives as the sol-gel aditive, and for separations of amino compounds where these changes varying by additive, analyte by analyte.
将三种广泛种植植物的乙醇提取物添加到硅溶胶 - 凝胶溶液中，随后将其用作内部石英毛细管的壁面改性剂。改性毛细管用于核苷酸和含氨基生物化合物（神经递质、氨基酸和寡肽）的开管毛细管电色谱分离。实验在生理pH值7.40下进行，并计算有效迁移率的最终变化。每种植物特有的特定化合物也作为溶胶 - 凝胶添加剂进行了测试，因此，在相同条件下，用如此改性的毛细管对相同的分析物进行分离。本研究的目的是找出流动体系中生理化合物与固定在溶胶 - 凝胶固定相中的常见植物提取物之间可能存在的相互作用。尽管每个毛细管中改性剂的量非常少，但基本统计评估显示，测试分析物的有效迁移率有一些不可忽略的变化。对于以姜黄、辣木或合成添加剂混合物作为溶胶 - 凝胶添加剂的毛细管中核苷酸的分离，以及对于氨基化合物的分离，这些变化大于±5%，且这些变化因添加剂和分析物而异。
