Back Florence, Mathieu Eric, Betscha Cosette, El Yakhlifi Salima, Arntz Youri, Ball Vincent
Faculté de Chirurgie Dentaire, Université de Strasbourg, 8 Rue Sainte Elisabeth, 67000 Strasbourg, France.
Unité Mixte de Recherche 1121, Institut National de la Santé et de la Recherche Médicale, 1 Rue Eugène Boeckel, CEDEX, 67084 Strasbourg, France.
Gels. 2022 Mar 31;8(4):210. doi: 10.3390/gels8040210.
The synthesis of surgical adhesives is based on the need to design glues that give rise to strong and fast bonds without cytotoxic side effects. A recent trend in surgical adhesives is to use gel-forming polymers modified with catechol groups, which can undergo oxidative crosslinking reactions and are strongly adhesive to all kinds on surfaces in wet conditions. We previously showed that blending gelatin with catechol can yield strong adhesion when the catechol is oxidized by a strong oxidant. Our previous work was limited to the study of the variation in the sodium periodate concentration. In this article, for an in-depth approach to the interactions between the components of the gels, the influence of the gelatin, the sodium periodate and dopamine/(pyro)catechol concentration on the storage (G') and loss (G″) moduli of the gels, as well as their adhesion on steel, have been studied by shear rheometry. The hydrogels were characterized by infrared and UV-Vis spectroscopy and the size of their pores visualized by digital microscopy and SEM after freeze drying but without further additives. In terms of adhesion between two stainless steel plates, the optimum was obtained for a concentration of 10% / in gelatin, 10 mM in sodium periodate, and 20 mM in phenolic compounds. Below these values, it is likely that crosslinking has not been maximized and that the oxidizing environment is weakening the gelatin. Above these values, the loss in adhesiveness may result from the disruption of the alpha helixes due to the large number of phenolic compounds as well as the maintenance of an oxidizing environment. Overall, this investigation shows the possibility to design strongly adhesive hydrogels to metal surfaces by blending gelatin with polyphenols in oxidative conditions.
外科粘合剂的合成基于设计出能产生牢固且快速粘合且无细胞毒性副作用的胶水的需求。外科粘合剂的一个最新趋势是使用经儿茶酚基团修饰的凝胶形成聚合物，其可发生氧化交联反应，并且在潮湿条件下对各种表面都具有很强的粘附性。我们之前表明，当儿茶酚被强氧化剂氧化时，将明胶与儿茶酚混合可产生很强的粘附力。我们之前的工作仅限于研究高碘酸钠浓度的变化。在本文中，为了深入研究凝胶成分之间的相互作用，通过剪切流变仪研究了明胶、高碘酸钠和多巴胺/（焦）儿茶酚浓度对凝胶储能模量（G'）和损耗模量（G''）的影响，以及它们在钢上的粘附力。通过红外光谱和紫外可见光谱对水凝胶进行了表征，并在冷冻干燥后但未添加其他添加剂的情况下，通过数字显微镜和扫描电子显微镜观察了其孔隙大小。就两块不锈钢板之间的粘附力而言，当明胶浓度为10%、高碘酸钠浓度为10 mM、酚类化合物浓度为20 mM时可获得最佳效果。低于这些值，交联可能未达到最大化，且氧化环境会使明胶变弱。高于这些值，粘附性的损失可能是由于大量酚类化合物导致α螺旋结构被破坏以及氧化环境的持续存在。总体而言，这项研究表明在氧化条件下将明胶与多酚混合设计出对金属表面具有强粘附性的水凝胶是有可能的。
