Ribeiro Cláudia, Santos Edgar T, Costa Luís, Brazinha Carla, Saraiva Pedro, Crespo João G
LAQV/Requimte, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.
A4F-Algae for Future, Campus do Lumiar, Estrada do Paço do Lumiar, Edif. E, R/C, 1649-038 Lisboa, Portugal.
Membranes (Basel). 2022 Apr 2;12(4):401. doi: 10.3390/membranes12040401.
This work proposes a way to maximize the potential of a sp. biorefinery process, through membrane technology, producing an extract enriched in soluble proteins, free from the insoluble protein fraction, with a low lipid content and eliminating the colored chlorophyll-a. This procedure, following the principles of a circular economy approach, allows for the valorization of a stream from the biorefining of sp. that, otherwise, would be considered a residue without commercial value. The process proposed minimizes fouling phenomena at the membrane surface, making it possible to achieve high permeate fluxes, thus reducing the need for membrane cleaning and, therefore, contributing to an extended membrane lifetime. Supernatant obtained after centrifugation of a suspension of ruptured sp. cells was processed by ultrafiltration using a membrane with a cut-off of 100 kDa MWCO. Two different operating approaches were evaluated-controlled transmembrane pressure and controlled permeate flux-under concentration and diafiltration modes. Ultrafiltration operated in a diafiltration mode, under controlled permeate flux conditions, led to the highest soluble protein recovery (78%) with the highest constant permeate flux (12 L·m·h) and low membrane fouling.
这项工作提出了一种方法，通过膜技术最大限度地发挥特定物种生物精炼过程的潜力，生产富含可溶性蛋白质的提取物，不含不溶性蛋白质部分，脂质含量低，并去除有色叶绿素-a。按照循环经济方法的原则，该程序能够使特定物种生物精炼过程中的一股物流增值，否则这股物流将被视为无商业价值的残渣。所提出的工艺将膜表面的污垢现象降至最低，从而能够实现高通量渗透，进而减少膜清洗的需求，因此有助于延长膜的使用寿命。将特定物种破裂细胞的悬浮液离心后得到的上清液，使用截留分子量为100 kDa的膜进行超滤处理。在浓缩和渗滤模式下，评估了两种不同的操作方法——控制跨膜压力和控制渗透通量。在控制渗透通量条件下以渗滤模式运行的超滤，实现了最高的可溶性蛋白质回收率（78%）、最高的恒定渗透通量（12 L·m·h）以及低膜污染。
