Teuling Emma, Wierenga Peter A, Schrama Johan W, Gruppen Harry
Aquaculture and Fisheries Group and ‡Laboratory of Food Chemistry, Wageningen University , Wageningen, The Netherlands.
J Agric Food Chem. 2017 Sep 13;65(36):7989-8002. doi: 10.1021/acs.jafc.7b01788. Epub 2017 Aug 28.
Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62% w/w) and in protein extractability (17-74% w/w), final protein isolates were obtained that had similar protein contents (62-77% w/w) and protein yields (3-9% w/w). Protein solubility as a function of pH was different between the sources and in ionic strength dependency, especially at pH < 4.0. Overall, the characterization and extraction protocol used allows a relatively fast and well-described isolation of purified proteins from novel protein sources.
光合单细胞生物被认为是很有前景的替代蛋白质来源。本研究的目的是了解这些绿色来源在总体组成方面的差异程度，以及这些差异如何影响最终的蛋白质分离物。使用温和的分离技术，从四种不同的单细胞来源（极大节旋藻（螺旋藻）、加的斯微拟球藻、透明四鞭藻和双形栅藻）中提取并分离蛋白质。尽管这些来源的蛋白质含量（27 - 62% w/w）和蛋白质可提取性（17 - 74% w/w）存在差异，但最终获得的蛋白质分离物具有相似的蛋白质含量（62 - 77% w/w）和蛋白质产率（3 - 9% w/w）。蛋白质溶解度作为pH的函数在不同来源之间以及在离子强度依赖性方面有所不同，尤其是在pH < 4.0时。总体而言，所使用的表征和提取方案能够相对快速且详细地从新型蛋白质来源中分离出纯化蛋白质。
