Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 11, A-1190 Wien, Austria; University of the Philippines Los Baños, College Laguna, Philippines.
Food Biotechnology Laboratory, Department of Food Science and Technology, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 11, A-1190 Wien, Austria; Doctoral Programme BioToP - Biomolecular Technology of Proteins, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 18, A-1190 Wien, Austria.
Bioelectrochemistry. 2020 Apr;132:107409. doi: 10.1016/j.bioelechem.2019.107409. Epub 2019 Nov 29.
Pyranose oxidase (POx) is an FAD-dependent oxidoreductase, and like glucose oxidase (GOx) it is a member of the glucose-methanol-choline (GMC) superfamily of oxidoreductases. POx oxidizes several monosaccharides including D-glucose, D-galactose, and D-xylose, while concurrently oxygen is reduced to hydrogen peroxide. In addition to this oxidase activity, POx shows pronounced activity with alternative electron acceptors that include various quinones or (complexed) metal ions. Even though POx in general shows properties that are more favourable than those of GOx (e.g., a considerably higher catalytic efficiency (k/K) for D-glucose, significantly lower Michaelis constants K for D-glucose, reactivity with both anomeric forms of D-glucose) it is much less frequently used for both biosensor and biofuel cell applications than GOx. POx has been applied in biosensing of D-glucose, D-galactose, and D-xylose, and in combination with α-glucosidase also maltose. An attractive application is in biosensors constructed for the measurement of 1,5-anhydro-D-glucitol, a recognised biomarker in diabetes. Bioelectrochemical applications of POx had been restricted to enzymes of fungal origin. The recent discovery and characterisation of POx from bacterial sources, which show properties that are very distinct from the fungal enzymes, might open new possibilities for further applications in bioelectrochemistry.
吡喃糖氧化酶（POx）是一种依赖黄素腺嘌呤二核苷酸（FAD）的氧化还原酶，与葡萄糖氧化酶（GOx）一样，它也是葡萄糖-甲醇-胆碱（GMC）氧化还原酶超家族的成员。POx 可氧化多种单糖，包括 D-葡萄糖、D-半乳糖和 D-木糖，同时氧气被还原为过氧化氢。除了这种氧化酶活性外，POx 还表现出与各种醌或（络合）金属离子等替代电子受体的显著活性。尽管 POx 通常表现出比 GOx 更有利的性质（例如，D-葡萄糖的催化效率（k/K）高得多，D-葡萄糖的米氏常数 K 低得多，对 D-葡萄糖的两种端基形式都有反应性），但它在生物传感器和生物燃料电池应用中比 GOx 要少得多。POx 已应用于 D-葡萄糖、D-半乳糖和 D-木糖的生物传感，以及与α-葡萄糖苷酶组合用于检测麦芽糖。一个有吸引力的应用是在用于测量 1,5-脱水-D-葡萄糖醇的生物传感器中，该物质是糖尿病的一种公认生物标志物。POx 的生物电化学应用仅限于真菌来源的酶。最近发现并表征了来自细菌来源的 POx，其性质与真菌酶有很大的不同，这可能为生物电化学的进一步应用开辟新的可能性。
