Ravoitytė Bazilė, Lukša Juliana, Wellinger Ralf Erik, Serva Saulius, Servienė Elena
Laboratory of Genetics, Institute of Botany, Nature Research Centre, Akademijos Str. 2, 08412 Vilnius, Lithuania.
Department of Biochemistry and Molecular Biology, Institute of Biosciences, Vilnius University, Saulėtekio Al. 7, 10257 Vilnius, Lithuania.
J Fungi (Basel). 2022 Apr 9;8(4):381. doi: 10.3390/jof8040381.
L-A virus is a widespread yeast dsRNA virus. The persistence of the L-A virus alone appears to be symptomless, but the concomitant presence of a satellite M virus provides a killer trait for the host cell. The presence of L-A dsRNA is common in laboratory, industrial, and wild yeasts, but little is known about the impact of the L-A virus on the host's gene expression. In this work, based on high-throughput RNA sequencing data analysis, the impact of the L-A virus on whole-genome expression in three different and host strains was analyzed. In the presence of the L-A virus, moderate alterations in gene expression were detected, with the least impact on respiration-deficient cells. Remarkably, the transcriptional adaptation of essential genes was limited to genes involved in ribosome biogenesis. Transcriptional responses to L-A maintenance were, nevertheless, similar to those induced upon stress or nutrient availability. Based on these data, we further dissected yeast transcriptional regulators that, in turn, modulate the cellular L-A dsRNA levels. Our findings point to totivirus-driven fine-tuning of the transcriptional landscape in yeasts and uncover signaling pathways employed by dsRNA viruses to establish the stable, yet allegedly profitless, viral infection of fungi.
L-A病毒是一种广泛存在的酵母双链RNA病毒。仅L-A病毒的持续存在似乎没有症状，但卫星M病毒的同时存在赋予宿主细胞杀伤特性。L-A双链RNA在实验室、工业和野生酵母中普遍存在，但关于L-A病毒对宿主基因表达的影响却知之甚少。在这项工作中，基于高通量RNA测序数据分析，研究了L-A病毒对三种不同宿主菌株全基因组表达的影响。在L-A病毒存在的情况下，检测到基因表达有适度变化，对呼吸缺陷型细胞的影响最小。值得注意的是，必需基因的转录适应性仅限于参与核糖体生物发生的基因。然而，对L-A维持的转录反应与应激或营养可利用性诱导的反应相似。基于这些数据，我们进一步剖析了酵母转录调节因子，这些调节因子反过来又调节细胞内L-A双链RNA水平。我们的研究结果表明，双链RNA病毒可对酵母转录图谱进行微调，并揭示了双链RNA病毒用于建立对真菌稳定但看似无利可图的病毒感染的信号通路。
