Kronfeld Hanna, Kemper Nicole, Hölzel Christina S
Department for Animal Hygiene and Animal Health, Institute of Breeding and Husbandry, Christian-Albrechts-University, Olshausenstr. 40, 24098 Kiel, Germany.
Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
Vet Sci. 2022 Apr 4;9(4):173. doi: 10.3390/vetsci9040173.
() forms part of the intestinal microbiome, but is also a known pathogen in histotoxic infections. The significance of the pathogen as a cause of uterine infections in cattle has been little studied so far. Here, we analyzed the association between a pathological puerperium in cattle and the detection of in a prospective longitudinal study. were only found in vaginal and uterine samples of diseased cattle, and were absent in healthy controls. Isolates ( = 21) were tested for the production of major toxins (alpha-, beta-, epsilon-toxin) by ELISA and for the potential of production of major (alpha-, beta-, iota-toxin) and minor toxins (beta2 toxin) by PCR. Furthermore, antimicrobial susceptibility was also tested phenotypically by microdilution. Despite the frequent use of tetracycline treatment in cows suffering from puerperal disorders, no isolate showed phenotypic tetracycline resistance. Most isolates did not release major amounts of toxin. The strict association of with puerperal disease, together with the absence of major toxins might hint towards a major role of other or unknown clostridial virulence factors in uterine disease.
（）是肠道微生物群的一部分，但也是组织毒性感染中已知的病原体。迄今为止，关于该病原体作为牛子宫感染病因的重要性研究甚少。在此，我们在一项前瞻性纵向研究中分析了牛病理性产褥期与（该病原体）检测之间的关联。（该病原体）仅在患病牛的阴道和子宫样本中被发现，在健康对照中未检测到。通过酶联免疫吸附测定（ELISA）检测了分离株（n = 21）主要毒素（α毒素、β毒素、ε毒素）的产生情况，并通过聚合酶链反应（PCR）检测了主要（α毒素、β毒素、ι毒素）和次要毒素（β2毒素）的产生潜力。此外，还通过微量稀释法对其抗菌敏感性进行了表型检测。尽管在患有产褥期疾病的奶牛中经常使用四环素治疗，但没有分离株表现出对四环素的表型耐药性。大多数分离株不会释放大量毒素。（该病原体）与产褥期疾病的严格关联，以及主要毒素的缺失，可能暗示其他或未知的梭菌毒力因子在子宫疾病中起主要作用。
