Parreira Valeria R, Russell Kay, Athanasiadou Spiridoula, Prescott John F
Department of Pathobiology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Disease Systems, Animal and Veterinary Sciences SRUC, Roslin Institute Building, Midlothian, EH25 9RG, Scotland.
BMC Microbiol. 2016 Aug 12;16(1):186. doi: 10.1186/s12866-016-0792-6.
Necrotic enteritis (NE) caused by netB-positive type A Clostridium perfringens is an important bacterial disease of poultry. Through its complex regulatory system, C. perfringens orchestrates the expression of a collection of toxins and extracellular enzymes that are crucial for the development of the disease; environmental conditions play an important role in their regulation. In this study, and for the first time, global transcriptomic analysis was performed on ligated intestinal loops in chickens colonized with a netB-positive C. perfringens strain, as well as the same strain propagated in vitro under various nutritional and environmental conditions.
Analysis of the respective pathogen transcriptomes revealed up to 673 genes that were significantly expressed in vivo. Gene expression profiles in vivo were most similar to those of C. perfringens grown in nutritionally-deprived conditions.
Taken together, our results suggest a bacterial transcriptome responses to the early stages of adaptation, and colonization of, the chicken intestine. Our work also reveals how netB-positive C. perfringens reacts to different environmental conditions including those in the chicken intestine.
由携带netB基因的A型产气荚膜梭菌引起的坏死性肠炎（NE）是家禽的一种重要细菌性疾病。通过其复杂的调控系统，产气荚膜梭菌协调一系列毒素和细胞外酶的表达，这些对于疾病的发展至关重要；环境条件在其调控中发挥重要作用。在本研究中，首次对定殖有携带netB基因的产气荚膜梭菌菌株的鸡的结扎肠段，以及在各种营养和环境条件下体外培养的同一菌株进行了全转录组分析。
对各自的病原体转录组分析显示，体内有多达673个基因显著表达。体内基因表达谱与在营养缺乏条件下生长的产气荚膜梭菌最为相似。
综合来看，我们的结果表明细菌转录组对鸡肠道适应和定殖早期阶段的反应。我们的工作还揭示了携带netB基因的产气荚膜梭菌如何对包括鸡肠道内环境在内的不同环境条件做出反应。
