Yang Yang, Miao Yunfeng, Zhong Shiwei, Fang Qiu, Wang Yiguang, Dong Bin, Zhao Hongbo
Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Hangzhou 311300, China.
Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Hangzhou 311300, China.
Plants (Basel). 2022 Apr 8;11(8):1015. doi: 10.3390/plants11081015.
is an aromatic plant which is widely used in landscaping and garden greening in China. However, the process of flower opening is significantly affected by ambient temperature changes. Cell expansion in petals is the primary factor responsible for flower opening. Xyloglucan endoglycolase/hydrolase (XTH) is a cell-wall-loosening protein involved in cell expansion or cell-wall weakening. Through whole-genome analysis, 38 genes were identified in which belong to the four main phylogenetic groups. The gene structure, chromosomal location, synteny relationship, and cis-acting elements prediction and expression patterns were analyzed on a genome-wide scale. The expression patterns showed that most were closely associated with the flower-opening period of . At the early flower-opening stage (S1 and S2), transcriptome and qRT-PCR analysis revealed the expression of , , , , and significantly increased under low ambient temperature (19 °C). It is speculated that the five genes might be involved in the regulation of flower opening by responding to ambient temperature changes. Our results provide solid foundation for the functional analysis of genes and help to explore the mechanism of flower opening responding to ambient temperature in .
是一种芳香植物，在中国广泛用于园林景观和庭院绿化。然而，花朵开放过程受到环境温度变化的显著影响。花瓣中的细胞扩张是花朵开放的主要因素。木葡聚糖内切糖苷酶/水解酶（XTH）是一种参与细胞扩张或细胞壁弱化的细胞壁松弛蛋白。通过全基因组分析，鉴定出38个属于四个主要系统发育组的基因。在全基因组范围内分析了基因结构、染色体定位、共线性关系、顺式作用元件预测和表达模式。表达模式表明，大多数基因与该植物的花期密切相关。在花朵开放早期阶段（S1和S2），转录组和qRT-PCR分析显示，在低温（19°C）下，某几个基因的表达显著增加。推测这五个基因可能通过响应环境温度变化参与花朵开放的调控。我们的结果为该植物基因的功能分析提供了坚实基础，并有助于探索该植物花朵开放对环境温度响应的机制。
