Halmagyi Adela, Vălimăreanu Sergiu, Șovărel Gabriela, Coste Ana
NIRDBS, Institute of Biological Research Cluj-Napoca, Republicii 48, 400015 Cluj-Napoca, Romania.
Department of Plant Molecular Biology, Faculty of Biology and Medicine, University of Lausanne, CH-1015 Lausanne, Switzerland.
Plants (Basel). 2022 Apr 18;11(8):1095. doi: 10.3390/plants11081095.
In this study, we compare two rapid cryopreservation (-196 °C) procedures, droplet-vitrification and encapsulation-dehydration for rose ( × L., cultivars 'Ioana', 'Mariana', 'Vulcan'). Significant factors for cryopreservation, such as sucrose concentration during osmoprotection, treatment duration with plant vitrification solution 2 (PVS2) in droplet-vitrification, duration of air desiccation and moisture content of alginate beads in encapsulation-dehydration, were investigated. In addition, the morphogenetic response to in vitro culture and to liquid nitrogen storage and the content in photosynthetic pigments have been assessed. The in vitro cultures were initiated from plant material originating from field collection. The highest regeneration frequencies were obtained for cv. 'Vulcan' in both of the cryopreservation procedures tested, 72% in droplet-vitrification and 65% following encapsulation-dehydration. The morphogenetic response (multiplication index and height of shoots) to liquid nitrogen storage was direct multiple shoot formation per initial shoot tip for all genotypes. The content in chlorophyll and was statistically comparable in plant material resulting from cryopreserved and non-cryopreserved shoot tips in all cultivars. The findings expand the information on 's response to in vitro culture conditions and cryopreservation, providing protocols with a high regeneration capacity for the storage of genotypes with high ornamental value.
在本研究中，我们比较了两种快速冷冻保存（-196°C）方法，即液滴玻璃化法和包囊脱水法，用于玫瑰（×L.，品种‘约安娜’‘玛丽安娜’‘瓦尔坎’）。研究了冷冻保存的重要因素，如渗透保护期间的蔗糖浓度、液滴玻璃化法中植物玻璃化溶液2（PVS2）的处理持续时间、包囊脱水法中的空气干燥持续时间和藻酸盐珠的水分含量。此外，还评估了对体外培养、液氮保存的形态发生反应以及光合色素含量。体外培养从田间采集的植物材料开始。在所测试的两种冷冻保存方法中，cv.‘瓦尔坎’均获得了最高的再生频率，液滴玻璃化法为72%，包囊脱水法为65%。对于所有基因型，对液氮保存的形态发生反应（增殖指数和芽高）是每个初始茎尖直接形成多个芽。在所有品种中，冷冻保存和未冷冻保存的茎尖产生的植物材料中叶绿素和的含量在统计学上具有可比性。这些发现扩展了关于对体外培养条件和冷冻保存反应的信息，为具有高观赏价值的基因型的保存提供了具有高再生能力的方案。
