Life Science Department-Invertebrate Zoology, Population Biology and Evolution / Fondation Faune Flore, Musée National d'Histoire Naturelle, 24, Rue Münster, 2160, Luxembourg, Luxembourg.
Sci Rep. 2022 Apr 21;12(1):6553. doi: 10.1038/s41598-022-10545-w.
LAMP assays are becoming increasingly popular in the field of invasive species detection but are still underused in eDNA-based monitoring. Here, we propose a LAMP assay designed to detect the North American crayfish species Pacifastacus leniusculus in water samples from streams. The presence of P. leniusculus was detected through this new LAMP assay in all but one of the nine sites sampled. No correlation was found between ddPCR absolute concentration measurements and the number of LAMP-positive technical replicates. However, we showed that using dependent technical replicates could significantly enhance the detection sensitivity of the LAMP assay. Applied to other assays, it could improve sensitivity and thus allow for a more efficient use of eDNA-based LAMP assays for invasive species detection in aquatic ecosystems.
LAMP 检测法在入侵物种检测领域越来越受欢迎，但在基于 eDNA 的监测中仍未得到充分利用。在这里，我们提出了一种 LAMP 检测法，旨在检测溪流水样中的北美淡水小龙虾（Pacifastacus leniusculus）。通过这种新的 LAMP 检测法，在采样的九个地点中，除了一个地点外，其他所有地点都检测到了 P. leniusculus 的存在。ddPCR 绝对浓度测量值与 LAMP 阳性技术重复数之间没有相关性。然而，我们表明，使用相关的技术重复可以显著提高 LAMP 检测法的检测灵敏度。将其应用于其他检测法中，可以提高灵敏度，从而使基于 eDNA 的 LAMP 检测法在水生生态系统中的入侵物种检测中更有效地发挥作用。
