Jothinarayanan Nivedhitha, Pham Chau Ha, Karlsen Frank, Roseng Lars Eric
Department of Microsystems, University of South-Eastern Norway, 3184 Raveien, Norway.
Methods Protoc. 2024 Oct 23;7(6):85. doi: 10.3390/mps7060085.
The monitoring of species in a habitat is important to ensure biological diversity. Environmental DNA (eDNA) can infer the presence-absence of species and enable rapid action to avoid threatening factors in ecosystems in the case of non-indigenous species. Loop-mediated isothermal amplification (LAMP) assays for molecular amplification are rapidly gaining popularity in species detection, but LAMP remains an underutilized method for eDNA-based monitoring practices. The most effective combination for successful species monitoring may be the collection of eDNA or biological traces collected by nanofiltration followed by LAMP-based species detection initiatives. Here, we used LAMP analysis to detect the eDNA of (Northern Pike), (European Eel), and (Atlantic Salmon) in Borre Lake and in the Drammen River. The selection of species is based on the categories of regionally invasive species, endangered species, and species of least concern. Two target genes were considered for each species and LAMP primers were designed. Our study showed that LAMP is an effective tool for discovering specific fish eDNA (analysis) to maintain aquatic ecosystems.
对栖息地中的物种进行监测对于确保生物多样性至关重要。环境DNA（eDNA）可以推断物种的存在与否，并在非本地物种的情况下促使采取快速行动以避免生态系统中的威胁因素。用于分子扩增的环介导等温扩增（LAMP）检测方法在物种检测中迅速受到欢迎，但LAMP在基于eDNA的监测实践中仍然是一种未得到充分利用的方法。成功进行物种监测的最有效组合可能是收集通过纳滤收集的eDNA或生物痕迹，然后开展基于LAMP的物种检测工作。在此，我们使用LAMP分析来检测博勒湖和德拉门河中白斑狗鱼、欧洲鳗鲡和大西洋鲑的eDNA。物种的选择基于区域入侵物种、濒危物种和最不受关注物种的类别。针对每个物种考虑了两个靶基因并设计了LAMP引物。我们的研究表明，LAMP是发现特定鱼类eDNA（进行分析）以维护水生生态系统的有效工具。
