Flandrois C, Gravagna B, Maire I, Mathieu M
Ann Biol Clin (Paris). 1986;44(5):486-90.
Study of enzymuria rises more and more interest in human pathology as a diagnosis parameter of renal diseases or as an index of nephrotoxicity. In this two fields, the literature demonstrated the interest of enzymes of the brush border of proximal tubuli (alanine amino peptidase, gamma glutamyl transpeptidase, alkaline phosphatase) and of lysosomal enzymes (beta glucuronidase and N acetyl beta D glucosaminidase). The authors analyse the problems limiting the present use of these methods: incomplete knowledge of enzyme of the different parts of the renal tissue and of the mechanisms of enzymuria (choice of enzymes and time periods for sampling); analytical problems referring to the study of enzymes in a complex medium (treatment and storage of samples, choice of adapted methods for activity measurement); at last the way of expression the results is still to be defined.
作为肾脏疾病的诊断参数或肾毒性指标，酶尿研究在人类病理学中越来越受到关注。在这两个领域，文献表明近端肾小管刷状缘酶（丙氨酸氨基肽酶、γ-谷氨酰转肽酶、碱性磷酸酶）和溶酶体酶（β-葡萄糖醛酸酶和N-乙酰-β-D-氨基葡萄糖苷酶）具有重要意义。作者分析了限制这些方法当前应用的问题：对肾组织不同部位酶以及酶尿机制（酶的选择和采样时间段）的了解不完整；在复杂介质中研究酶时涉及的分析问题（样品的处理和储存、选择合适的活性测量方法）；最后，结果的表达方法仍有待确定。
