Wang Tao, Zhang Derui, Yang Boming, Su Nana, Cui Jin
College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
College of Agriculture, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2022 Apr 5;13:848454. doi: 10.3389/fpls.2022.848454. eCollection 2022.
Indole-3-carbinol (I3C), an important secondary metabolite with strong anti-cancer ability, is widely found in cruciferous plants. Light and phytohormones are one of the most important external and internal signals, respectively, that control the growth, development, and secondary metabolism of the plant life cycle. However, there are few studies about the influence of the blue light and salicylic acid (SA) on the regulation of I3C accumulation. In this study, a negative correlation was found between the content of I3C and SA in different species. Among this, broccoli and were chosen for further studies. We observed that blue light treatment increased the accumulation of I3C, and exogenous SA treatment significantly inhibited the accumulation of I3C in broccoli sprouts. Based on the RNA sequence, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that blue light promoted the enrichment of differentially expressed genes (DEGs) in plant hormone signal transduction pathways. More specifically, downregulated expression of genes related to SA biosynthesis and upregulated expression of I3C genes related to metabolic pathway were observed under blue light. Taken together, these results suggested that SA negatively regulates blue light-induced I3C accumulation in broccoli sprouts.
吲哚 - 3 - 甲醇（I3C）是一种具有强大抗癌能力的重要次生代谢产物，广泛存在于十字花科植物中。光和植物激素分别是控制植物生命周期的生长、发育和次生代谢的最重要的外部和内部信号之一。然而，关于蓝光和水杨酸（SA）对I3C积累调控的影响的研究很少。在本研究中，发现不同物种中I3C含量与SA之间存在负相关。其中，选择西兰花和[此处原文缺失信息]进行进一步研究。我们观察到蓝光处理增加了I3C的积累，而外源SA处理显著抑制了西兰花芽苗中I3C的积累。基于RNA序列，京都基因与基因组百科全书（KEGG）通路分析表明，蓝光促进了植物激素信号转导通路中差异表达基因（DEG）的富集。更具体地说，在蓝光下观察到与SA生物合成相关的基因表达下调，与代谢途径相关的I3C基因表达上调。综上所述，这些结果表明SA负调控蓝光诱导的西兰花芽苗中I3C的积累。
