Laboratory of Biochemistry and Biomedical Materials, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Department of Laboratory Medicine, Qingdao Central Hospital, Second Affiliated Hospital of Qingdao University, Qingdao 266042, PR China.
Carbohydr Polym. 2022 Jul 15;288:119329. doi: 10.1016/j.carbpol.2022.119329. Epub 2022 Mar 15.
Third-degree scald, causing serious tissue destruction with continuous pain, easily leads to microbial infections and delayed wound healing. Therefore, a multifunctional treatment is attractive for seriously damaged tissue. Herein, carboxymethyl chitosan-coordinated argentum (Ag-CMC) was synthesized via a complexation method, and then the Ag release, antibacterial activity, biocompatibility, pain relief and wound healing properties of Ag-CMC were investigated in vitro and in vivo. The results revealed that Ag had interacted with carboxymethyl chitosan, containing approximately 1.2% of silver. The Ag-CMC (50-200 μg/mL) with Ag sustained release exhibited significant antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, drug-resistant E. coli, PA, MRSA and good biocompatibility with L929 cells. Furthermore, antibacterial and wound healing experiments demonstrated that Ag-CMC achieved an effective contraction rate of 90% after 28 days by accelerating re-epithelialization, regulating inflammation response, relieving pain and infections. Therefore, Ag-CMC is a safe multifunctional treatment for wound healing and infections.
三度烫伤，可导致严重的组织破坏和持续疼痛，容易引发微生物感染和伤口愈合延迟。因此，多功能治疗对于严重受损的组织具有吸引力。本文通过配位法合成了羧甲基壳聚糖配位银（Ag-CMC），并在体外和体内研究了 Ag-CMC 的 Ag 释放、抗菌活性、生物相容性、缓解疼痛和伤口愈合性能。结果表明，Ag 与含有约 1.2%银的羧甲基壳聚糖相互作用。Ag-CMC（50-200μg/mL）具有持续释放 Ag 的功能，对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、白色念珠菌、耐药大肠杆菌、PA、MRSA 具有显著的抗菌活性，对 L929 细胞具有良好的生物相容性。此外，抗菌和伤口愈合实验表明，Ag-CMC 通过加速再上皮化、调节炎症反应、缓解疼痛和感染，在 28 天后达到 90%的有效收缩率。因此，Ag-CMC 是一种安全的多功能治疗方法，可用于伤口愈合和感染。
