College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China; Shandong Engineering Research Center for Marine Environment Corrosion and Safety Protection, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China; Shandong Engineering Technology Research Center for Advanced Coating, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China; Shandong Engineering Research Center for Marine Environment Corrosion and Safety Protection, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China; Shandong Engineering Technology Research Center for Advanced Coating, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
Int J Biol Macromol. 2021 Apr 1;175:481-494. doi: 10.1016/j.ijbiomac.2021.02.045. Epub 2021 Feb 8.
In this paper, Ag-Metal-organic framework loaded chitosan nanoparticles (0.1%Ag@MOF/1.5%CSNPs) and polyvinyl alcohol/sodium alginate/chitosan (PACS) were used as the upper and lower layers to successfully prepare a bilayer composite dressing for wound healing. The performance of bilayer dressing was evaluated. The lower layer (PACS) had uniform pore size distribution, good water retention, swelling, water vapor permeability, and biocompatibility while PACS had almost no antibacterial activity. The upper layer (Ag@MOF/CSNPs) possessed excellent antibacterial activity and poor biocompatibility. As the upper layer, it can avoid direct contact with the skin and inhibit microbial invasion. In addition, the bilayer can adhere to a large number of red blood cells and platelets, promoting blood coagulation and cell proliferation. Ag@MOF, CSNPs, Ag@MOF/CSNPs and bilayer showed antibacterial activity in ascending order, due to the synergistic antibacterial action of the upper and lower layer. In vivo evaluation showed that both bilayer and PACS could significantly accelerate the wound healing, and the bilayer dressing showed more complete re-epithelialization with less inflammatory cells. In summary, this new bilayer composite is an ideal dressing for accelerating wound healing.
本文中，载银金属-有机骨架壳聚糖纳米粒子（0.1%Ag@MOF/1.5%CSNPs）和聚乙烯醇/海藻酸钠/壳聚糖（PACS）被分别用作上下两层，成功制备了用于伤口愈合的双层复合敷料。评估了双层敷料的性能。下层（PACS）具有均匀的孔径分布、良好的保水、溶胀、水蒸气透过性和生物相容性，而 PACS 几乎没有抗菌活性。上层（Ag@MOF/CSNPs）具有优异的抗菌活性和较差的生物相容性。作为上层，它可以避免与皮肤直接接触，抑制微生物入侵。此外，双层敷料可以黏附大量的红细胞和血小板，促进血液凝固和细胞增殖。Ag@MOF、CSNPs、Ag@MOF/CSNPs 和双层敷料的抗菌活性依次递增，这是由于上下层的协同抗菌作用。体内评价表明，双层敷料和 PACS 均能显著促进伤口愈合，双层敷料的再上皮化更完整，炎症细胞更少。综上所述，这种新型双层复合敷料是一种加速伤口愈合的理想敷料。
