LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica, Portugal.
Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica, Portugal.
J Appl Microbiol. 2022 Aug;133(2):656-664. doi: 10.1111/jam.15587. Epub 2022 May 4.
We aim to explore the non-structural sugars from white wine grape pomace (WWGP) as the input carbon source for the co-production of multiple high-value products by the non-fastidious yeast Rhodotorula babjevae to create a sustainable and economically appealing process.
Water extraction of unfermented, soluble sugars from WWGP yielded extracts with similar amounts of glucose and fructose, which were used to prepare a growth medium. Rhodorotula babjevae multiplied as fast on WWGP-based medium as on a reference medium but achieved higher cell dry weight (CDW) and lower intracellular triacylglycerol accumulation (22.5% vs. 28.6%) in WWGP-based medium. In addition, R. babjevae produced mannitol and arabitol and carotenoids and secreted polyol esters of fatty acids, a rare type of glycolipid as confirmed by Fourier transform-infrared, nuclear magnetic resonance and high-performance liquid chromatography analyses. Remarkably, R. babjevae consumed simultaneously both fructose and glucose when on WWGP-based medium and left glucose practically untouched in the reference medium, evidencing a fructophilic character.
Rhodorotula babjevae, a metabolic versatile yeast, proliferated on a minimally processed extract and successfully converted glucose and fructose into high-value products.
Different chemicals with market potential can be produced through the valorization of abundant waste feedstocks generated by the wine industry to which R. babjevae can contribute.
我们旨在探索白葡萄酒葡萄渣（WWGP）中的非结构性糖作为非严格酵母罗德里西亚 babjevae 的共同生产多种高价值产品的输入碳源，以创造可持续和经济上有吸引力的工艺。
WWGP 中未发酵的可溶性糖的水提取产生了具有相似葡萄糖和果糖含量的提取物，这些提取物被用于制备生长培养基。罗德里西亚 babjevae 在 WWGP 为基础的培养基上的繁殖速度与参考培养基一样快，但在 WWGP 为基础的培养基中实现了更高的细胞干重（CDW）和更低的细胞内三酰基甘油积累（22.5%对 28.6%）。此外，R. babjevae 产生甘露醇和阿拉伯糖醇以及类胡萝卜素，并分泌脂肪酸多元醇酯，这是一种罕见的糖脂类型，通过傅里叶变换红外、核磁共振和高效液相色谱分析得到证实。值得注意的是，当在 WWGP 为基础的培养基上时，R. babjevae 同时消耗果糖和葡萄糖，而在参考培养基中几乎不消耗葡萄糖，证明其具有果糖偏好性。
罗德里西亚 babjevae 是一种代谢多功能酵母，在经过最少加工的提取物上增殖，并成功地将葡萄糖和果糖转化为高价值产品。
通过对葡萄酒工业产生的丰富废料进行增值利用，可以生产出具有市场潜力的不同化学品，而 R. babjevae 可以为此做出贡献。
