Institute for Genetics of Prokaryotes, Faculty of Biology and CeBiTec, Bielefeld University, 33615 Bielefeld, Germany.
Molecules. 2023 Feb 20;28(4):1996. doi: 10.3390/molecules28041996.
Circular economy holds great potential to minimize the use of finite resources, and reduce waste formation by the creation of closed-loop systems. This also pertains to the utilization of sidestreams in large-scale biotechnological processes. A flexible feedstock concept has been established for the industrially relevant , which naturally synthesizes the yellow C50 carotenoid decaprenoxanthin. In this study, we aimed to use a preprocessed aquaculture sidestream for production of carotenoids, including the fish feed ingredient astaxanthin by . The addition of a preprocessed aquaculture sidestream to the culture medium did not inhibit growth, obviated the need for addition of several components of the mineral salt's medium, and notably enhanced production of astaxanthin by an engineered producer strain. Improved astaxanthin production was scaled to 2 L bioreactor fermentations. This strategy to improve astaxanthin production was shown to be transferable to production of several native and non-native carotenoids. Thus, this study provides a proof-of-principle for improving carotenoid production by upon supplementation of a preprocessed aquaculture sidestream. Moreover, in the case of astaxanthin production it may be a potential component of a circular economy in aquaculture.
循环经济具有很大的潜力，可以通过创建闭环系统来最小化有限资源的使用，并减少废物的形成。这也适用于大规模生物技术过程中副产物的利用。已经建立了一种用于工业相关的灵活原料概念，它可以自然合成黄色 C50 类胡萝卜素脱叶叶黄素。在这项研究中，我们旨在使用预处理的水产养殖副产物流来生产类胡萝卜素，包括作为鱼饲料成分的虾青素。向培养基中添加预处理的水产养殖副产物不仅没有抑制生长，而且还避免了添加几种盐培养基成分的需要，并显著提高了工程化的 生产菌的虾青素产量。改进的虾青素生产已扩大到 2 L 生物反应器发酵。事实证明，这种提高虾青素产量的策略可以转移到几种天然和非天然类胡萝卜素的生产中。因此，本研究提供了一个通过添加预处理的水产养殖副产物来提高 的类胡萝卜素生产的原理验证。此外，就虾青素生产而言，它可能是水产养殖循环经济的一个潜在组成部分。
