• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

不同细胞培养基对CD8+ T细胞扩增的影响：一项生物信息学研究

The Impact of Different Cell Culture Mediums on CD8+ T Cells Expansion: A Bioinformatics Study.

作者信息

Jalili Arsalan, Hajifathali Abbas, Bereimipour Ahmad, Roshandel Elham, Aghdami Nasser

机构信息

Department of Applied Cell Sciences, Faculty of Basic Sciences and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran.

Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.

出版信息

Cell J. 2022 Mar;24(3):155-162. doi: 10.22074/cellj.2022.7779.

DOI:10.22074/cellj.2022.7779
PMID:35451586
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9035229/
Abstract

OBJECTIVE

Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signaling pathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select the best candidate.

MATERIALS AND METHODS

In this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis.

RESULTS

The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosome dynamics, telomeres, telomerase, and cellular aging signal pathways.

CONCLUSION

Using bioinformatics analyzes, integrated and regular genes were selected as common genes and inhibitor, interleukin 7 and 15 expansion media.

摘要

目的

不同的细胞培养基可影响T细胞的扩增。本研究旨在评估在不同常用T细胞扩增培养基中培养的T细胞中的信号通路、蛋白质相互作用和基因，以选择最佳候选物。

材料与方法

在这项计算机模拟观察研究中，利用生物信息学分析和富集数据库，通过微阵列分析研究基因表达谱。

结果

本研究结果共同筛选出26个上调基因和59个下调基因，这些基因参与了SREBP对脂质合成的控制、T细胞激活有丝分裂和染色体动力学过程中的共刺激信号、端粒、端粒酶以及细胞衰老信号通路。

结论

通过生物信息学分析，选择整合且规律的基因作为共同基因以及抑制剂、白细胞介素7和15扩增培养基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed51/9035229/64349e0cd709/Cell-J-24-155-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed51/9035229/65897831d544/Cell-J-24-155-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed51/9035229/a10fbb92c074/Cell-J-24-155-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed51/9035229/b840ed897e51/Cell-J-24-155-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed51/9035229/64349e0cd709/Cell-J-24-155-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed51/9035229/65897831d544/Cell-J-24-155-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed51/9035229/a10fbb92c074/Cell-J-24-155-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed51/9035229/b840ed897e51/Cell-J-24-155-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed51/9035229/64349e0cd709/Cell-J-24-155-g04.jpg

相似文献

1
The Impact of Different Cell Culture Mediums on CD8+ T Cells Expansion: A Bioinformatics Study.不同细胞培养基对CD8+ T细胞扩增的影响：一项生物信息学研究
Cell J. 2022 Mar;24(3):155-162. doi: 10.22074/cellj.2022.7779.
2
IL-15 Upregulates Telomerase Expression and Potently Increases Proliferative Capacity of NK, NKT-Like, and CD8 T Cells.白细胞介素-15上调端粒酶表达并显著增强自然杀伤细胞、自然杀伤T细胞样细胞和CD8 T细胞的增殖能力。
Front Immunol. 2021 Jan 18;11:594620. doi: 10.3389/fimmu.2020.594620. eCollection 2020.
3
Preferential T cell expansion by artificial antigen presenting cells expressing 4-1BBL alone or in combination with CD80 or CD86.单独表达4-1BBL或与CD80或CD86联合表达的人工抗原呈递细胞对T细胞的优先扩增。
Iran J Immunol. 2008 Sep;5(3):136-47.
4
Co-stimulation with 4-1BB ligand allows extended T-cell proliferation, synergizes with CD80/CD86 and can reactivate anergic T cells.与4-1BB配体共刺激可使T细胞增殖延长，与CD80/CD86协同作用，并可重新激活无反应性T细胞。
Int Immunol. 2007 Dec;19(12):1383-94. doi: 10.1093/intimm/dxm106. Epub 2007 Oct 31.
5
Bioinformatics analysis of key genes and signaling pathways associated with myocardial infarction following telomerase activation.端粒酶激活后与心肌梗死相关的关键基因和信号通路的生物信息学分析。
Mol Med Rep. 2017 Sep;16(3):2915-2924. doi: 10.3892/mmr.2017.6938. Epub 2017 Jul 6.
6
Tumor suppressive effects of WEE1 gene silencing could not enhance immunopotentiation effects of CD80 and 4-1BBL co-stimulation in human T cells.WEE1基因沉默的肿瘤抑制作用不能增强CD80和4-1BBL共刺激在人T细胞中的免疫增强作用。
J Cancer Res Ther. 2015 Oct-Dec;11(4):708-16. doi: 10.4103/0973-1482.147746.
7
Bioinformatics analysis and identification of genes and molecular pathways in steroid-induced osteonecrosis of the femoral head.生物信息学分析及鉴定激素性股骨头坏死相关基因和分子通路。
J Orthop Surg Res. 2021 May 20;16(1):327. doi: 10.1186/s13018-021-02464-9.
8
Changes in lymphocytes' telomerase activity by 4-1BB costimulation.4-1BB共刺激对淋巴细胞端粒酶活性的影响。
J Cancer Res Ther. 2014 Oct-Dec;10(4):998-1003. doi: 10.4103/0973-1482.137906.
9
Ex vivo T lymphocyte expansion for retroviral transduction: influence of serum-free media on variations in cell expansion rates and lymphocyte subset distribution.用于逆转录病毒转导的离体T淋巴细胞扩增：无血清培养基对细胞扩增速率变化和淋巴细胞亚群分布的影响。
Exp Hematol. 2000 Oct;28(10):1137-46. doi: 10.1016/s0301-472x(00)00526-9.
10
Integrated bioinformatics analysis for differentially expressed genes and signaling pathways identification in gastric cancer.胃癌差异表达基因及信号通路的综合生物信息学分析。
Int J Med Sci. 2021 Jan 1;18(3):792-800. doi: 10.7150/ijms.47339. eCollection 2021.

本文引用的文献

1
Generation of Antitumor T Cells For Adoptive Cell Therapy With Artificial Antigen Presenting Cells.利用人工抗原呈递细胞生成用于过继性细胞治疗的抗肿瘤T细胞。
J Immunother. 2020 Apr;43(3):79-88. doi: 10.1097/CJI.0000000000000306.
2
CD8 T cells regulate tumour ferroptosis during cancer immunotherapy.CD8 T 细胞在癌症免疫治疗中调节肿瘤铁死亡。
Nature. 2019 May;569(7755):270-274. doi: 10.1038/s41586-019-1170-y. Epub 2019 May 1.
3
Adoptive T Cell Therapy Strategies for Viral Infections in Patients Receiving Haematopoietic Stem Cell Transplantation.
造血干细胞移植患者病毒感染的过继细胞治疗策略。
Cells. 2019 Jan 14;8(1):47. doi: 10.3390/cells8010047.
4
Inhibition of AKT signaling uncouples T cell differentiation from expansion for receptor-engineered adoptive immunotherapy.抑制 AKT 信号通路可将受体工程化过继免疫疗法中的 T 细胞分化与扩增脱耦联。
JCI Insight. 2017 Dec 7;2(23):95103. doi: 10.1172/jci.insight.95103.
5
Monocyte-Derived Dendritic Cells with Silenced PD-1 Ligands and Transpresenting Interleukin-15 Stimulate Strong Tumor-Reactive T-cell Expansion.沉默 PD-1 配体和转染白细胞介素-15 的单核细胞衍生树突状细胞刺激强烈的肿瘤反应性 T 细胞扩增。
Cancer Immunol Res. 2017 Aug;5(8):710-715. doi: 10.1158/2326-6066.CIR-16-0336. Epub 2017 Jun 21.
6
Ex vivo Akt inhibition promotes the generation of potent CD19CAR T cells for adoptive immunotherapy.体外 Akt 抑制促进了高效 CD19CAR T 细胞的生成，用于过继免疫治疗。
J Immunother Cancer. 2017 Mar 21;5:26. doi: 10.1186/s40425-017-0227-4. eCollection 2017.
7
IL-15 promotes activation and expansion of CD8+ T cells in HIV-1 infection.白细胞介素-15在HIV-1感染中促进CD8 + T细胞的激活和扩增。
J Clin Invest. 2016 Jul 1;126(7):2745-56. doi: 10.1172/JCI85996. Epub 2016 Jun 20.
8
Regulation of T cells by mTOR: the known knowns and the known unknowns.mTOR对T细胞的调控：已知与未知
Trends Immunol. 2015 Jan;36(1):13-20. doi: 10.1016/j.it.2014.11.005. Epub 2014 Dec 16.
9
Inhibition of Akt signaling promotes the generation of superior tumor-reactive T cells for adoptive immunotherapy.抑制Akt信号传导可促进生成用于过继性免疫治疗的优质肿瘤反应性T细胞。
Blood. 2014 Nov 27;124(23):3490-500. doi: 10.1182/blood-2014-05-578583. Epub 2014 Oct 21.
10
Differences in the transduction of canonical Wnt signals demarcate effector and memory CD8 T cells with distinct recall proliferation capacity.经典Wnt信号转导的差异区分了具有不同回忆增殖能力的效应性和记忆性CD8 T细胞。
J Immunol. 2014 Sep 15;193(6):2784-91. doi: 10.4049/jimmunol.1400465. Epub 2014 Aug 15.