不同细胞培养基对CD8+ T细胞扩增的影响：一项生物信息学研究
The Impact of Different Cell Culture Mediums on CD8+ T Cells Expansion: A Bioinformatics Study.
作者信息
Jalili Arsalan, Hajifathali Abbas, Bereimipour Ahmad, Roshandel Elham, Aghdami Nasser
机构信息
Department of Applied Cell Sciences, Faculty of Basic Sciences and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran.
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
出版信息
Cell J. 2022 Mar;24(3):155-162. doi: 10.22074/cellj.2022.7779.
OBJECTIVE
Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signaling pathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select the best candidate.
MATERIALS AND METHODS
In this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis.
RESULTS
The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosome dynamics, telomeres, telomerase, and cellular aging signal pathways.
CONCLUSION
Using bioinformatics analyzes, integrated and regular genes were selected as common genes and inhibitor, interleukin 7 and 15 expansion media.
目的
不同的细胞培养基可影响T细胞的扩增。本研究旨在评估在不同常用T细胞扩增培养基中培养的T细胞中的信号通路、蛋白质相互作用和基因，以选择最佳候选物。
材料与方法
在这项计算机模拟观察研究中，利用生物信息学分析和富集数据库，通过微阵列分析研究基因表达谱。
结果
本研究结果共同筛选出26个上调基因和59个下调基因，这些基因参与了SREBP对脂质合成的控制、T细胞激活有丝分裂和染色体动力学过程中的共刺激信号、端粒、端粒酶以及细胞衰老信号通路。
结论
通过生物信息学分析，选择整合且规律的基因作为共同基因以及抑制剂、白细胞介素7和15扩增培养基。
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