Département de Pharmacologie Clinique et Toxicologie, Université de Lorraine, CHRU-Nancy, 54500, Vandœuvre, France.
Université de Lorraine, CNRS, IMoPA, 54505, Vandœuvre, France.
Anal Bioanal Chem. 2022 Jun;414(14):4203-4215. doi: 10.1007/s00216-022-04073-9. Epub 2022 Apr 22.
Oral fluid is easy and safe to collect and allows the detection of drugs of abuse after local exposure by oral, smoked, and/or inhaled intake, or systemic exposure. A routine online solid-phase extraction UPLC-MS/MS method was developed for the simultaneous determination of 33 psychoactive drugs in oral fluid. The selected drugs were fourteen fentanyl analogs and nineteen other abused psychoactive compounds, including classical narcotics, which were analyzed in a run of 10 min. Limits of detection and of quantification ranged from 0.02 to 1 ng/mL and from 0.02 to 5 ng/mL depending on the analyte, respectively. Matrix effect was in the range - 17 to + 15.7% for all analytes having a deuterated analog. Accuracy ranged from 82.7 to 113.4% and precision CV was at worst of 18.6%. Carryover was below 0.8% for all analytes. Recovery from FLOQSwabs™ showed high variability between analytes with THC, D2FF, 4-ANPP, ocfentanil, and valerylfentanyl being recovered below 40%. A stability study performed over 2 weeks on collecting devices loaded with artificial oral fluid showed huge variation between analytes with morphine, BZE, and norfentanyl being the more stable. Storage at 4 °C allowed drug detection for 1 week except for THC and remifentanil. The method was successfully applied to the detection of abused psychoactive compounds in oral fluid samples from 6 patients admitted to an addiction department.
唾液采集简便、安全，可用于检测经口腔、吸食或吸入摄入或全身暴露后滥用药物。本研究建立了一种在线固相萃取超高效液相色谱-串联质谱法，用于同时检测唾液中的 33 种精神活性药物。所选药物包括 14 种芬太尼类似物和 19 种其他滥用精神活性化合物，包括经典麻醉剂，可在 10 分钟内完成分析。根据分析物的不同，检测限和定量限分别在 0.02-1ng/mL 和 0.02-5ng/mL 范围内。所有分析物的基质效应在-17%至+15.7%之间，均有氘代类似物。准确度在 82.7%至 113.4%之间，精密度 CV 最差为 18.6%。所有分析物的交叉污染均低于 0.8%。FLOQSwabs™ 从唾液采集装置中的回收率在分析物之间差异很大，THC、D2FF、4-ANPP、ocfentanil 和 valerylfentanyl 的回收率低于 40%。在装有人工唾液的采集装置上进行了为期 2 周的稳定性研究，结果显示分析物之间存在巨大差异，其中吗啡、BZE 和去甲芬太尼较为稳定。4°C 下储存可在 1 周内检测到药物，除了 THC 和雷米芬太尼。该方法成功应用于检测 6 名成瘾科患者唾液样本中的滥用精神活性化合物。
