大鼠后肢动脉供血不足的研究。体内骨骼肌血流与葡萄糖利用的相关性。

An investigation of arterial insufficiency in the rat hindlimb. Correlation of skeletal muscle bloodflow and glucose utilization in vivo.

作者信息

Challiss R A, Hayes D J, Radda G K

出版信息

Biochem J. 1986 Dec 1;240(2):395-401. doi: 10.1042/bj2400395.

DOI:10.1042/bj2400395

PMID:3545186

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1147431/

Abstract

Muscle bloodflow and the rate of glucose uptake and phosphorylation were measured in vivo in rats 7 days after unilateral femoral artery ligation and section. Bloodflow was determined by using radiolabelled microspheres. At rest, bloodflow to the gastrocnemius, plantaris and soleus muscles of the ligated limb was similar to their respective mean contralateral control values; however, bilateral sciatic nerve stimulation at 1 Hz caused a less pronounced hyperaemic response in the muscles of the ligated limb, being 59, 63 and 49% of their mean control values in the gastrocnemius, plantaris and soleus muscles respectively. The rate of glucose utilization was determined by using the 2-deoxy[3H]glucose method [Ferré, Leturque, Burnol, Penicaud & Girard (1985) Biochem. J. 228, 103-110]. At rest, the rate of glucose uptake and phosphorylation was statistically significantly increased in the gastrocnemius and soleus muscles of the ligated limb, being 126 and 140% of the mean control values respectively. Bilateral sciatic nerve stimulation at 1 Hz caused a 3-5-fold increase in the rate of glucose utilization by the ligated and contralateral control limbs; furthermore, the rate of glucose utilization was significantly increased in the muscles of the ligated limb, being 140, 129 and 207% of their mean control values respectively. For the range of bloodflow to normally perfused skeletal muscle at rest or during isometric contraction determined in the present study, a linear correlation between the rate of glucose utilization and bloodflow can be demonstrated. Applying similar methods of regression analysis to glucose utilization and bloodflow to muscles of the ligated limb reveals a similar linear correlation. However, the rate of glucose utilization at a given bloodflow is increased in muscles of the ligated limb, indicating an adaptation of skeletal muscle to hypoperfusion.

摘要

在单侧股动脉结扎和切断后 7 天的大鼠体内，测量了肌肉血流量以及葡萄糖摄取和磷酸化速率。血流量通过使用放射性标记微球来测定。静息时，结扎肢体的腓肠肌、跖肌和比目鱼肌的血流量与其各自对侧对照平均值相似；然而，以 1Hz 频率双侧刺激坐骨神经时，结扎肢体肌肉的充血反应不那么明显，腓肠肌、跖肌和比目鱼肌的充血反应分别为其对照平均值的 59%、63%和 49%。葡萄糖利用率通过 2-脱氧[3H]葡萄糖法测定[费雷、勒图尔克、布尔诺尔佩尼考德和吉拉尔（1985 年）《生物化学杂志》228 卷，103 - 110 页]。静息时，结扎肢体的腓肠肌和比目鱼肌中葡萄糖摄取和磷酸化速率在统计学上显著增加，分别为对照平均值的 126%和 140%。以 1Hz 频率双侧刺激坐骨神经使结扎肢体和对侧对照肢体的葡萄糖利用率增加 3 - 5 倍；此外，结扎肢体肌肉中的葡萄糖利用率显著增加，分别为其对照平均值的 140%、129%和 207%。对于本研究中测定的静息或等长收缩时正常灌注骨骼肌的血流量范围，可以证明葡萄糖利用率与血流量之间存在线性相关性。将类似的回归分析方法应用于结扎肢体肌肉的葡萄糖利用率和血流量，也显示出类似的线性相关性。然而，在给定血流量下，结扎肢体肌肉中的葡萄糖利用率增加，表明骨骼肌对低灌注有适应性变化。