Challiss R A, Hayes D J, Petty R F, Radda G K
Biochem J. 1986 Jun 1;236(2):461-7. doi: 10.1042/bj2360461.
A small animal model of arterial insufficiency is presented which involves unilateral femoral artery ligation and section. Invoked alterations in metabolism and perfusion of the affected muscle mass have been investigated 12 h, 4, 7 and 14 days post-ligation by 31P-n.m.r. and microsphere infusion, both at rest and during isometric muscle contraction at 1 Hz. At rest, the concentration of phosphocreatine was similar to the mean control value (36.0 +/- 1.0 mM) from 4 days post-ligation, but was significantly lower at 12 h (28.5 +/- 3.6 mM). Inorganic phosphate concentrations were significantly elevated for 7 days post-ligation. No significant differences were noted in intramuscular pH. Upon stimulation of the affected muscle mass, a time-dependent improvement in phosphocreatine utilization was observed such that 14 days post-ligation phosphocreatine utilization was not significantly different from mean control values. A similar amelioration was noted for the contraction-induced fall in intramuscular pH. At rest, no significant differences in bloodflow to the muscles of the ligated limb compared with the unaffected contralateral limb were observed. However, isometric contraction of the affected muscle mass resulted in a markedly reduced hyperaemic response 12 h post-ligation. Thereafter, a time-dependent improvement in tissue perfusion during stimulation was observed which paralleled the improvements in phosphocreatine utilization and intramuscular pH changes. The results presented are discussed with respect to the interrelationship between oxygen delivery, high energy phosphate utilization and force maintenance.
本文介绍了一种小动物动脉供血不足模型，该模型涉及单侧股动脉结扎和切断。在结扎后12小时、4天、7天和14天，通过31P核磁共振和微球灌注技术，对受影响肌肉块的代谢和灌注变化进行了研究，研究分别在静息状态和1Hz等长肌肉收缩过程中进行。静息状态下，结扎后4天磷酸肌酸浓度与平均对照值（36.0±1.0 mM）相似，但在12小时时显著降低（28.5±3.6 mM）。结扎后7天内无机磷酸盐浓度显著升高。肌肉内pH值无显著差异。刺激受影响的肌肉块后，观察到磷酸肌酸利用随时间的改善，结扎后14天磷酸肌酸利用与平均对照值无显著差异。收缩诱导的肌肉内pH值下降也有类似改善。静息状态下，与未受影响的对侧肢体相比，结扎肢体肌肉的血流量无显著差异。然而，结扎后12小时，受影响肌肉块的等长收缩导致充血反应明显降低。此后，观察到刺激过程中组织灌注随时间的改善，这与磷酸肌酸利用和肌肉内pH值变化的改善情况平行。本文就氧输送、高能磷酸盐利用和力量维持之间的相互关系对所呈现的结果进行了讨论。
