Bianco J A, Bakanauskas J, Carlson M, Jones S, Moring A, Alpert J S, Klassen V
Department of Nuclear Medicine, University of Massachusetts Medical Center, Worcester 01605.
Eur J Nucl Med. 1988;13(11):557-62. doi: 10.1007/BF02574767.
We studied the cardiac uptake of 2-14C-D-deoxyglucose (14C-DG) in 6 dogs subjected to a 15 min left anterior descending coronary artery (LAD) occlusion followed by 30 min reflow. Coronary arterial flows during occlusion were determined with 46Sc microspheres. After 30 min reflow, 50 microCi 14C-DG were given intravenously to determine cardiac uptake. The animal was killed 45 min later and 46Sc counts/min were determined in gamma and beta counters. Quenching was determined with non radioactive blood for both isotopes in the beta counter. Contribution of 46Sc counts/min to the 14C channel was subtracted. 14C uptake was in DPM/g and was higher in the cardiac ischemic regions of four of the six dogs. In three of the six animals there was an inverse curvilinear relation between 14C-DG cardiac uptake and occlusion flow. This observation was further confirmed by high resolution autoradiography, indicating that enhanced cardiac uptake of radiolabeled DG may be a useful positron emission tomographic marker for reversible myocardial ischemia.
我们研究了6只犬在左前降支冠状动脉（LAD）闭塞15分钟后再灌注30分钟过程中2-¹⁴C-D-脱氧葡萄糖（¹⁴C-DG）的心脏摄取情况。用⁴⁶Sc微球测定闭塞期间的冠状动脉血流。再灌注30分钟后，静脉注射50微居里的¹⁴C-DG以测定心脏摄取。45分钟后处死动物，在γ和β计数器中测定⁴⁶Sc计数/分钟。在β计数器中用非放射性血液测定两种同位素的淬灭情况。减去⁴⁶Sc计数/分钟对¹⁴C通道的贡献。¹⁴C摄取以每分钟衰变数/克表示，在6只犬中的4只的心脏缺血区域较高。在6只动物中的3只，¹⁴C-DG心脏摄取与闭塞血流之间存在反向曲线关系。高分辨率放射自显影进一步证实了这一观察结果，表明放射性标记的DG心脏摄取增强可能是可逆性心肌缺血的一种有用的正电子发射断层扫描标志物。
