Asgari Mehrdad, Abdollahifar Mohammad-Amin, Gazor Rouhallah, Salmani Tayyebali, Khosravipour Armin, Mahmoudi Yaser, Baniasadi Farzad, Hamblin Michael R, Abrahamse Heidi, Chien Sufan, Bayat Mohammad
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Anatomy, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Photobiomodul Photomed Laser Surg. 2022 Apr;40(4):261-272. doi: 10.1089/photob.2021.0127.
This study examined the use of photobiomodulation (PBM) plus adipose-derived stem cells (ASCs) to enhance the osteogenic properties of demineralized bone matrix (DBM) scaffold in a critical size femoral defect (CSFD) of ovariectomy-induced osteoporotic (OVX) rats. PBM could be used as a unique strategy to enhance the osteogenic potential of DBMs seeded with ASCs. The OVX rats with a CSFD were divided into six groups: (1) Control (C); (2) DBM scaffold alone (S); (3) S+PBM; (4) S+alendronate; (5) S+ASC; (6) S+PBM+ASC. Stereological analysis, real-time polymerase chain reaction (RT-PCR), and cone-beam computed tomography (CBCT) were performed after euthanization at 4 and 8 weeks postimplantation surgery. In the 8th week, Groups 4 and 6 showed a greatly high new trabecular bone volume than the scaffold group (all, = 0.009). The CBCT data demonstrated that the CSFD was significantly smaller in the two, three, and six groups relative to the control group ( = 0.01, = 0.000, and = 0.000, respectively). RT-PCR revealed that Groups 3 and 6 had higher messenger RNA levels of osteocalcin (OC) and osteoprotegerin (OPG) compared with the control group ( = 0.05). Group 6 had significantly lower expression of receptor activator of nuclear factor-κB ligand (RANKL) compared with the control group ( = 0.02). The combination of DBM plus PBM plus ASC, as well as DBM plus PBM significantly improved the healing of CSFD in OVX rats, and affected the expression of OPG, OC, and RANKL genes.
本研究检测了光生物调节作用（PBM）联合脂肪来源干细胞（ASCs）对去卵巢诱导骨质疏松（OVX）大鼠临界尺寸股骨缺损（CSFD）中脱矿骨基质（DBM）支架成骨特性的增强作用。PBM可作为一种独特策略来增强接种了ASCs的DBM的成骨潜能。将患有CSFD的OVX大鼠分为六组：（1）对照组（C）；（2）单独使用DBM支架（S）；（3）S+PBM；（4）S+阿仑膦酸钠；（5）S+ASC；（6）S+PBM+ASC。在植入手术后4周和8周处死后进行体视学分析、实时聚合酶链反应（RT-PCR）和锥形束计算机断层扫描（CBCT）。在第8周时，第4组和第6组的新小梁骨体积比支架组高得多（均为，=0.009）。CBCT数据表明，相对于对照组，第2、3和6组的CSFD明显更小（分别为=0.01，=0.000，和=0.000）。RT-PCR显示，与对照组相比，第3组和第6组的骨钙素（OC）和骨保护素（OPG）信使核糖核酸水平更高（=0.05）。与对照组相比，第6组核因子κB受体活化因子配体（RANKL）的表达明显更低（=0.02）。DBM联合PBM联合ASC以及DBM联合PBM显著改善了OVX大鼠CSFD的愈合，并影响了OPG、OC和RANKL基因的表达。
