Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Biomedical section, University of Cagliari, Cittadella Universitaria di Monserrato, 09042 Monserrato, Cagliari, Italy.
Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Marine Bioecology section, University of Cagliari, via T. Fiorelli 1, 09126 Cagliari, Italy.
Biol Open. 2022 May 15;11(5). doi: 10.1242/bio.059019. Epub 2022 May 13.
Vitellogenin is an essential protein involved in ovary maturation in many animals. Detection of this protein correlated with reproductive capacity may be important if carried out on marine organisms such as the red spiny lobster Palinurus elephas, a crustacean that is an economically important crop from wild fish catches. Moreover, in recent years, vitellogenin has assumed an important role as a possible biomarker of marine environmental pollution, as its expression levels can be influenced by the presence of similar estrogen pollutants and can affect the reproductive sphere of marine organisms such as crustaceans. The P. elephas vitellogenin protein and its coding gene have never been isolated, so there is little information about its presence in this lobster. The aim of the present study was to develop a molecular strategy to create, for the first time, an antibody for the detection and quantization of vitellogenin in P. elephas.
卵黄蛋白原是一种在许多动物的卵巢成熟过程中起关键作用的蛋白质。如果对海洋生物（如红刺龙虾 Palinurus elephas）进行检测，这种与生殖能力相关的蛋白质可能很重要，因为红刺龙虾是一种从野生鱼类捕捞中获得的具有经济重要性的甲壳类动物。此外，近年来，卵黄蛋白原作为海洋环境污染的一种潜在生物标志物的作用越来越重要，因为其表达水平可能受到类似雌激素污染物的影响，并可能影响甲壳类等海洋生物的生殖领域。然而，P. elephas 的卵黄蛋白原蛋白及其编码基因从未被分离出来，因此关于这种龙虾中存在卵黄蛋白原的信息很少。本研究的目的是开发一种分子策略，首次为 P. elephas 中的卵黄蛋白原创建一种用于检测和定量的抗体。
