Yan Xu, Chen Yucai, Ma Li, Liu Yongchun, Qi Yu, Liu Sijin
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Antioxidants (Basel). 2022 Apr 10;11(4):754. doi: 10.3390/antiox11040754.
There are still significant concerns about the detrimental effects and health risks of particulate matters (PMs) on the respiratory system. Notably, a largely overlooked knowledge gap is whether the environmental ageing process would change the physicochemical properties of PMs as well as the toxic influences of PMs on macrophages. Here, we applied ambient treatment of model PMs to mimic the real O-induced ageing process and investigated ageing-determined cytotoxicity profile changes of PMs towards macrophages. The consequent distinct bioreactivity and toxicity towards macrophages are largely attributed to the changes of species of surface O-functional groups. Importantly, we unveiled the specific interactions between aged PMs and macrophages due to the variant contents of the surface carboxyl group, resulting in the divergent inflammatory activations and immune balance in the lung. Collectively, this study unearths the significance of ageing in altering particle cytotoxicity, and also provides additional understandings for consecutive investigations on the adverse effects of air pollution on the respiratory system.
人们仍然非常关注颗粒物（PMs）对呼吸系统的有害影响和健康风险。值得注意的是，一个很大程度上被忽视的知识空白是环境老化过程是否会改变PMs的物理化学性质以及PMs对巨噬细胞的毒性影响。在这里，我们对模型PMs进行环境处理以模拟真实的氧化诱导老化过程，并研究了老化决定的PMs对巨噬细胞的细胞毒性谱变化。随之而来的对巨噬细胞明显不同的生物反应性和毒性很大程度上归因于表面氧官能团种类的变化。重要的是，我们揭示了由于表面羧基含量的变化，老化的PMs与巨噬细胞之间的特定相互作用，导致肺部不同的炎症激活和免疫平衡。总的来说，这项研究揭示了老化在改变颗粒细胞毒性方面的重要性，也为空气污染对呼吸系统的不利影响的后续研究提供了更多的认识。
